Identification of ERF genes in peanuts and functional analysis of AhERF008 and AhERF019 in abiotic stress response

Wan, L and Wu, Y and Huang, J and Dai, X and Lei, Y and Yan, L and Jiang, H and Zhang, J and Varshney, R K and Liao, B (2014) Identification of ERF genes in peanuts and functional analysis of AhERF008 and AhERF019 in abiotic stress response. Functional and Integrative Genomics, 14 (3). pp. 467-477. ISSN 1438-793X

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Abstract

Ethylene-responsive factor (ERF) play an important role in regulating gene expression in plant development and response to stresses. In peanuts (Arachis hypogaea L.), which produce flowers aerially and pods underground, only a few ERF genes have been identified so far. This study identifies 63 ERF unigenes from 247,313 peanut EST sequences available in the NCBI database. The phylogeny, gene structures, and putative conserved motifs in the peanut ERF proteins were analysed. Comparative analysis revealed the absence of two subgroups (A1 and A3) of the ERF family in peanuts; only 10 subgroups were identified in peanuts compared to 12 subgroups in Arabidopsis and soybeans. AP2/ERF domains were found to be conserved among peanuts, Arabidopsis, and soybeans. Outside the AP2/ERF domain, many soybean-specific conserved motifs were also detected in peanuts. The expression analysis of ERF family genes representing each clade revealed differential expression patterns in response to biotic and abiotic stresses. Overexpression of AhERF008 influenced the root gravity of Arabidopsis, whereas overexpression of AhERF019 enhanced tolerance to drought, heat, and salt stresses in Arabidopsis. The information generated in this study will be helpful to further investigate the function of ERFs in plant development and stress response.

Item Type:	Article
Divisions:	RP-Grain Legumes
CRP:	CGIAR Research Program on Grain Legumes
Uncontrolled Keywords:	ERFfamily. Genefunction. Phylogeny. Peanut. Stress response. Plant development. Phylogeny, Peanut, Stress response, Plant development,
Subjects:	Mandate crops > Groundnut Others > Genetics and Genomics Others > Plant Physiology
Depositing User:	Mr Siva Shankar
Date Deposited:	11 Jun 2014 04:09
Last Modified:	13 Oct 2014 08:38
URI:	http://oar.icrisat.org/id/eprint/8109
Official URL:	http://dx.doi.org/10.1007/s10142-014-0381-4
Projects:	UNSPECIFIED
Funders:	National Natural Science Foundation of China, National Basic Research Program of China, China Agriculture Research System, China Postdoctoral Science Foundation
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

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