Experimental studies on pollen-mediated gene flow in Sorghum bicolor (L.) Moench using male-sterile bait plants

Rabbi, I Y and Parzies, H K and Kiambi, D and Haussmann, B I G and Folkertsma, R and Giger, H (2011) Experimental studies on pollen-mediated gene flow in Sorghum bicolor (L.) Moench using male-sterile bait plants. Plant Breeding, 130 (2). pp. 217-224.

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Abstract

Information on the potential of pollen mediated gene flow (PMGF) in sorghum is required for ensuring varietal purity and to mitigate risk transgenic gene flow. Replicated trials were conducted in Kenya using a local landrace, 'Ochuti' as pollen donor surrounded by male-sterile pollen baits. Frequency of PMGF decreased with the increase of distance from pollen sources and was significantly influenced by wind direction and speed. Anther dehiscence correlated with increase in vapour pressure deficit in the morning. A negative exponential regression model with logarithmic transformation of PMGF and square-root transformation of distance from source field best fitted the data. Up to 50% of female florets on a male sterile (MS) plant were pollinated at 1 m from pollen source and declined to 14% at 10 m. The maximum distance of PMGF using the PMGF model, based on a threshold of one seed per MS plant, was 203 m when data above the 95th percentile is considered. However, in the presence of self-produced pollen of male-fertile target plants, the possibility of long-distance cross-pollination may be very low.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	sorghum; pollen dispersal; gene flow; flowering characteristics
Agro Tags:	<b>Agrotags</b> - sorghum | planting | crops | genes | winds | genetics | farms | experimentation | seasons | transgenics <br><b>Fishtags</b> - NOT-AVAILABLE<br><b>Geopoliticaltags</b> - kenya | africa | ethiopia | maine | china | texas | centre
Subjects:	Mandate crops > Sorghum
Depositing User:	Siva Shankar
Date Deposited:	30 May 2011 08:25
Last Modified:	10 Jun 2011 09:12
URI:	http://oar.icrisat.org/id/eprint/53
Official URL:	http://dx.doi.org/10.1111/j.1439-0523.2010.01775.x
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	This work is part of the PhD thesis of the first author who was funded by a Deutscher Akademischer Austausch Dienst (DAAD) scholarship in the framework of the project �Arresting the scourge of Striga in Africa by combining the strengths of marker-assisted selection and farmer participatory breeding of farmer preferred sorghum varieties� funded by the Bundesministerium fu¨ r Wirtschaftliche Zusammenarbeit und Entwicklung (BMZ), Bonn. We sincerely wish to thank Joseph Muange, Mohammed Somo, and all the staff of the International Crops Research Institute for the Semi-Arid Tropics, Kiboko Field Station, Kenya, for their excellent technical assistance during the field experiments. We thank the anonymous reviewers who provided tremendous help by through important, accurate, and constructive suggestions for making this manuscript more comprehensible.
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