Hima Kumari, P and Anil Kumar, S and Sivan, P and Katam, R and Suravajhala, P and Rao, K S and Varshney, R K and Kavi Kishor, P B (2017) Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis. Frontiers in Plant Science, 7 (2027). pp. 1-15. ISSN 1664-462X
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Abstract
A Na+/H+ antiporter-like protein (NHXLP) was isolated from Sorghum bicolor L. (SbNHXLP) and validated by overexpressing in tomato for salt tolerance. Homozygous T2 transgenic lines when evaluated for salt tolerance, accumulated low Na+ and displayed enhanced salt tolerance compared to wild-type plants (WT). This is consistent with the amiloride binding assay of the protein. Transgenics exhibited higher accumulation of proline, K+, Ca2+, improved cambial conductivity, higher PSII, and antioxidative enzyme activities than WT. Fluorescence imaging results revealed lower Na+ and higher Ca2+ levels in transgenic roots. Co-immunoprecipitation experiments demonstrate that SbNHXLP interacts with a Solanum lycopersicum cation proton antiporter protein2 (SlCHX2). qRT-PCR results showed upregulation of SbNHXLP and SlCHX2 upon treatment with 200 mM NaCl and 100 mM potassium nitrate. SlCHX2 is known to be involved in K+ acquisition, and the interaction between these two proteins might help to accumulate more K+ ions, and thus maintain ion homeostasis. These results strongly suggest that plasma membrane bound SbNHXLP involves in Na+ exclusion, maintains ion homeostasis in transgenics in comparison with WT and alleviates NaCl stress.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Genetic Gains |
| CRP: | CGIAR Research Program on Grain Legumes |
| Uncontrolled Keywords: | SbNHXLP, tomato, salt stress, co-immunoprecipitation, CHX2 |
| Subjects: | Others > Genetics and Genomics |
| Depositing User: | Mr Ramesh K |
| Date Deposited: | 31 Mar 2017 09:31 |
| Last Modified: | 18 Apr 2017 05:57 |
| URI: | http://oar.icrisat.org/id/eprint/9931 |
| Official URL: | http://dx.doi.org/10.3389/fpls.2016.02027 |
| Projects: | UNSPECIFIED |
| Funders: | Department of Science and Technology |
| Acknowledgement: | This study was supported by grants from the Department of Science and Technology (DST No: SR/SO/PS-55/07), New Delhi. PHK is thankful to the DST and UGC for providing fellowship. PBK is grateful to the CSIR, New Delhi, for providing Emeritus-fellowship.We thank Dr. Rajagopal, University of Hyderabad, Hyderabad for permitting us to use Handy plant efficiency analyzer. |
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