Genome-wide development and deployment of informative intron-spanning and intron-length polymorphism markers for genomics-assisted breeding applications in chickpea

Srivastava, R and Bajaj, D and Sayal, Y K and Meher, P K and Upadhyaya, H D and Kumar, R and Tripathi, S and Bharadwaj, C and Rao, A R and Parida, S K (2016) Genome-wide development and deployment of informative intron-spanning and intron-length polymorphism markers for genomics-assisted breeding applications in chickpea. Plant Science, 252. pp. 374-387. ISSN 01689452

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Abstract

The discovery and large-scale genotyping of informative gene-based markers is essential for rapid delineation of genes/QTLs governing stress tolerance and yield component traits in order to drive genetic enhancement in chickpea. A genome-wide 119169 and 110491 ISM (intron-spanning markers) from 23129 desi and 20386 kabuli protein-coding genes and 7454 in silico InDel (insertion-deletion) (1–45-bp)-based ILP (intron-length polymorphism) markers from 3283 genes were developed that were structurally and functionally annotated on eight chromosomes and unanchored scaffolds of chickpea. A much higher amplification efficiency (83%) and intra-specific polymorphic potential (86%) detected by these markers than that of other sequence-based genetic markers among desi and kabuli chickpea accessions was apparent even by a cost-effective agarose gel-based assay. The genome-wide physically mapped 1718 ILP markers assayed a wider level of functional genetic diversity (19–81%) and well-defined phylogenetics among domesticated chickpea accessions. The gene-derived 1424 ILP markers were anchored on a high-density (inter-marker distance: 0.65 cM) desi intra-specific genetic linkage map/functional transcript map (ICC 4958 × ICC 2263) of chickpea. This reference genetic map identified six major genomic regions harbouring six robust QTLs mapped on five chromosomes, which explained 11–23% seed weight trait variation (7.6–10.5 LOD) in chickpea. The integration of high-resolution QTL mapping with differential expression profiling detected six including one potential serine carboxypeptidase gene with ILP markers (linked tightly to the major seed weight QTLs) exhibiting seed-specific expression as well as pronounced up-regulation especially in seeds of high (ICC 4958) as compared to low (ICC 2263) seed weight mapping parental accessions. The marker information generated in the present study was made publicly accessible through a user-friendly web-resource, “Chickpea ISM-ILP Marker Database”. The designing of multiple ISM and ILP markers (2–5 markers/gene) from an individual gene (transcription factor) with numerous aforementioned desirable genetic attributes can widen the user-preference to select suitable primer combination for simultaneous large-scale assaying of functional allelic variation, natural allelic diversity, molecular mapping and expression profiling of genes among chickpea accessions. This will essentially accelerate the identification of functionally relevant molecular tags regulating vital agronomic traits for genomics-assisted crop improvement by optimal resource expenses in chickpea.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	CGIAR Research Program on Grain Legumes
Uncontrolled Keywords:	Genetic map; ILP; ISM; Marker; QTL; Chickpea
Subjects:	Others > Plant Breeding Mandate crops > Chickpea Others > Genetics and Genomics
Depositing User:	Mr Ramesh K
Date Deposited:	09 Jan 2017 06:30
Last Modified:	17 May 2017 10:28
URI:	http://oar.icrisat.org/id/eprint/9850
Official URL:	http://dx.doi.org/10.1016/j.plantsci.2016.08.013
Projects:	UNSPECIFIED
Funders:	Department of Biotechnology (DBT)
Acknowledgement:	The authors gratefully acknowledge the financial sup-port for this study provided by a research grant from the Department of Biotechnology (DBT), Government of India(102/IFD/SAN/2161/2013-14). We are thankful to the Editor andreviewers for critically evaluating the manuscript and providing constructive comments.
Links:	Google Scholar

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