Enumeration of rhizobia by enzyme-linked immunosorbent assay (ELISA)

Nambiar, P T C and Anjaiah, V (2008) Enumeration of rhizobia by enzyme-linked immunosorbent assay (ELISA). Journal of Applied Bacteriology, 58 (2). pp. 187-193. ISSN 00218847

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Abstract

The use of the enzyme-linked immunosorbent assay (ELISA) to enumerate rhizobia in peat carrier and in soil has been investigated. The ELISA technique takes less time than the conventional plant infection technique often used to enumerate rhizobia present in the presence of other micro-organisms. A minimum of 102–103 cells are required for a detectable ELISA reaction, limiting the use of this technique when the number of rhizobia is low.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Rhizobia
Subjects:	Others
Depositing User:	Mr Ramesh K
Date Deposited:	14 Sep 2016 06:02
Last Modified:	14 Sep 2016 06:02
URI:	http://oar.icrisat.org/id/eprint/9666
Official URL:	http://dx.doi.org/10.1111/j.1365-2672.1985.tb01446...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors thank R. Rajeshari and N. Bharathan for their help in conducting ELISA tests and Barry L. Noh and Judith A. Kipe-Nolt for their critical comments on the manuscript.
Links:	Google Scholar

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