Ali, R and Hash, C T and Damris, O and Elhussein, A and Mohamed, A H (2016) Introgression of striga resistance into popular Sudanese sorghum varieties using marker assisted selection. World Journal of Biotechnology, 01 (01). pp. 48-55. ISSN 2518-0878
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Abstract
Witchweed (Strigaspp.) is one of the most important cereals production constraints globally and is projected to worsen with anticipated climate change. It is especially a devastating parasitic weed in Sub-Saharan Africa and parts of Asia. Integrated management strategies that depend mainly on host plant resistance provide the most effective control mechanism for Striga. We used molecular marker-assisted backcrossing to introgress Striga resistance from a resistant genotype, N13, into agronomically important genetic backgrounds (Tabat, Wad and Ahmed). Backcross populations BC3S3 were generated and genotyped using Simple Sequence Repeat (SSR) and Diversity Arrays Technology (DArT) markers. A total of 17 promising backcross progenies were selected and screened in Striga infested field alongside their parents. The Area Under Striga Progress Curve (AUSPC) showed significant decrease in Striga count (920-7.5) resulting in a 97-189% increase in yield under Striga pressure. Our results demonstrate the practical application of marker assisted selection (MAS) to generate farmer-preferrd Striga resistant lines in Sudan.
| Item Type: | Article |
|---|---|
| Divisions: | RP-Dryland Cereals |
| CRP: | CGIAR Research Program on Dryland Cereals |
| Uncontrolled Keywords: | Witchweed, QTL, MAS, SSR and DArT, AUSPC, Strigaspp, Cereals production, Sudan |
| Subjects: | Mandate crops > Sorghum |
| Depositing User: | Mr Ramesh K |
| Date Deposited: | 15 Jun 2016 06:53 |
| Last Modified: | 21 Oct 2016 05:02 |
| URI: | http://oar.icrisat.org/id/eprint/9551 |
| Official URL: | http://dx.doi.org/10.1234/wjb.v1i1.34 |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | The authors would like to thank the Association for Strengthening Agricultural Research in Eastern and Central Africa, (ASARECA), International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) and Biosciences eastern and central Africa (BecA-ILRI Hub), ABCF fellowship, for providing financial support for this work. |
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