Ssr-based molecular profiling of selected donors of wide compatibility, elongated uppermost internode, stigma exsertion and submergence tolerance traits and parental lines of commercial rice (o. Sativa l.) Hybrids

Priyadarshi, R and Singh, A K and Khandekar, D and Koradi, P and DeumuduNaidu, P and Khera, P and Hariprasad, A S and Ulaganathan, K and Shenoy, V (2015) Ssr-based molecular profiling of selected donors of wide compatibility, elongated uppermost internode, stigma exsertion and submergence tolerance traits and parental lines of commercial rice (o. Sativa l.) Hybrids. International Journal of Agricultural Science and Research (IJASR), 5 (2). pp. 67-92. ISSN 2321-0087

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Abstract

Molecular breeding plays an important role in sustainable agriculture development. Hybrid rice technology aims to increase the yield potential of rice beyond the level of inbred high-yielding varieties (HYVs) by exploiting the phenomenon of hybrid vigour or heterosis. Improvement of hybrid rice parental line is necessary to meet the food security problem. Parental polymorphism was carried with 215 SSR markers between five recurrents and ten donors. During the foreground selection, both reported markers (S5-Indel and BF-S5) were validated for wide compatibility, 2 out of 14 (ART5 and SC3) validates for submergence tolerance, one out of two (RM5) validate for stigma exsertion, whereas 2 of 3 markers (RM5970, RM3476) validated for elongated uppermost internode traits between recurrents and donors. For background selection, maximum polymorphic markers (112) between IR58025eB i.e improved maintainer line with elongated uppermost internode and Oryza meridionalis and minimum polymorphic markers (42) between IR79156B and IR91-1591-3 were found. Marker-assisted backcrossing accelerate, the transfer of gene of interest in desirable genetic background. Genotypes IR58025B and IR58025eB emerged as genetically most similar with a value of 97%. The genotypes IR64 Sub1 and Oryza meridionalis were found most divergent showing 33% genetic similarity. Dissimilarity coefficient of the generated information obtained on genetic relatedness would be supportive in further rice breeding program.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Backcrossing, Parental Polymorphism, Simple Sequence Repeats (SSR), Genetic Diversity
Subjects: Others > Food Legumes
Others > Food and Nutrition
Depositing User: Mr Ramesh K
Date Deposited: 07 Aug 2015 11:01
Last Modified: 07 Aug 2015 11:02
URI: http://oar.icrisat.org/id/eprint/8911
Official URL: http://www.tjprc.org/view-archives.php?year=2015&j...
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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