Kumar, C S and Sireesha, K and Ranga Rao, G V and Reddy, A S and Waliyar, F and Rambabu, C and Kumar, P L (2007) Production of polyclonal antibodies for detection of Nucleopolyhedrovirus infecting Helicoverpa armigera. Indian Journal of Plant Protection, 35 (2). pp. 210-212.
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Abstract
Polyclonal antibodies were raised against purified polyhedrin [polyocclusion body (POB)] protein preparations of Helicoverpa armigera nucleopolyhedrovirus (HaNPV) and were used to develop a direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA) to detect HaNPV. The sensitivity of the DAC-ELISA was 15 ng/ml of partially purified viral protein or 30 ng/ml POBs from the HaNPV infected larval extracts. The antibodies were highly specific to polyhedrin protein of HaNPV and were useful to diagnose NPV at early stages of larval infection and also for the quantification of the NPVs during production of viral insecticides.
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Others > Agriculture-Farming, Production, Technology, Economics |
| Depositing User: | Library ICRISAT |
| Date Deposited: | 05 Sep 2011 00:42 |
| Last Modified: | 05 Sep 2011 00:42 |
| URI: | http://oar.icrisat.org/id/eprint/882 |
| Official URL: | |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
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