Biological control of Botrytis cinerea and plant growth promotion potential by Penicillium citrinum in chickpea (Cicer arietinum L.)

Sreevidya, M and Gopalakrishnan, S and Melo, T M and Simic, N and Bruheim, P and Sharma, M and Srinivas, V and Alekhya, G (2015) Biological control of Botrytis cinerea and plant growth promotion potential by Penicillium citrinum in chickpea (Cicer arietinum L.). Biocontrol Science and Technology, 25 (7). pp. 739-755. ISSN 0958-3157

PDF - Published Version Restricted to ICRISAT users only Download (357kB) | Request a copy

Abstract

A total of 48 fungi were characterised for their antagonistic potential against Botrytis cinerea causing Botrytis Gray Mold (BGM) disease in chickpea by dual culture and metabolite production assays. The culture filtrate of the most promising isolate, VFI-51, was purified by various chromatographic techniques and identified as ‘citrinin’ by nuclear magnetic resonance and mass spectrometry studies. The efficacy of citrinin was demonstrated to control BGM in chickpea under greenhouse conditions. The sequences of 18S rDNA gene of the VFI-51 matched with Penicillium citrinum in BLAST analysis. The VFI-51 produced siderophore, hydrocyanic acid, indole-3-acetic acid, lipase, protease and β-1,3- glucanase; grew well in NaCl (up to 15%), at pH between 7 and 11 and temperatures between 20°C and 40°C; and was compatible with fungicides bavistin and thiram. Under greenhouse and field conditions, VFI-51 significantly enhanced the nodule number, nodule weight, root and shoot weight and stover and grain yield over the un-inoculated control. In the rhizosphere, VFI-51 also significantly enhanced total N, available P and OC over the un-inoculated control. Scanning electron microscopy analysis revealed that VFI-51 colonised on the roots of chickpea. This study concluded that VFI-51 has the potential for biocontrol of BGM and plant growth promotion in chickpea.

Item Type:	Article
Divisions:	RP-Grain Legumes
CRP:	CGIAR Research Program on Grain Legumes
Uncontrolled Keywords:	Penicillium citrinum; citrinin; biocontrol; Botrytis Gray Mold; plant growth promotion; chickpea
Subjects:	Mandate crops > Chickpea
Depositing User:	Mr B K Murthy
Date Deposited:	31 Mar 2015 08:37
Last Modified:	21 Oct 2016 06:41
URI:	http://oar.icrisat.org/id/eprint/8627
Official URL:	http://dx.doi.org/10.1080/09583157.2015.1010483
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

View Statistics

Actions (login required)

View Item