Marker-assisted introgression of a QTL region to improve rust resistance in three elite and popular varieties of peanut (Arachis hypogaea L.)

    Varshney, R K and Pandey, M K and Janila, P and Nigam, S N and Sudini, H and Gowda, M V C and Sriswathi, M and Radhakrishnan, T and Manohar, S S and Nagesh, P (2014) Marker-assisted introgression of a QTL region to improve rust resistance in three elite and popular varieties of peanut (Arachis hypogaea L.). Theoretical and Applied Genetics, 127 (8). pp. 1771-1781. ISSN 0040-5752

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    Abstract

    Leaf rust, caused by Puccinia arachidis Speg, is one of the major devastating diseases in peanut (Arachis hypogaea L.). One QTL region on linkage group AhXV explaining upto 82.62 % phenotypic variation for rust resistance was validated and introgressed from cultivar ‘GPBD 4’ into three rust susceptible varieties (‘ICGV 91114’, ‘JL 24’ and ‘TAG 24’) through marker-assisted backcrossing (MABC). The MABC approach employed a total of four markers including one dominant (IPAHM103) and three co-dominant (GM2079, GM1536, GM2301) markers present in the QTL region. After 2–3 backcrosses and selfing, 200 introgression lines (ILs) were developed from all the three crosses. Field evaluation identified 81 ILs with improved rust resistance. Those ILs had significantly increased pod yields (56–96 %) in infested environments compared to the susceptible parents. Screening of selected 43 promising ILs with 13 markers present on linkage group AhXV showed introgression of the target QTL region from the resistant parent in 11 ILs. Multi-location field evaluation of these ILs should lead to the release of improved varieties. The linked markers may be used in improving rust resistance in peanut breeding programmes.

    Item Type: Article
    Divisions: RP-Grain Legumes
    CRP: CGIAR Research Program on Grain Legumes
    Subjects: Mandate crops > Groundnut
    Others > Genetics and Genomics
    Depositing User: Mr Siva Shankar
    Date Deposited: 16 Jun 2014 04:09
    Last Modified: 21 Nov 2014 06:31
    URI: http://oar.icrisat.org/id/eprint/8128
    Official URL: http://dx.doi.org/10.1007/s00122-014-2338-3
    Projects: Tropical Legumes I & II projects
    Funders: Bill and Melinda Gates Foundation
    Acknowledgement: Authors are thankful to Bryan Moss and V. Papaiah for extending their technical assistance. Thanks are also due to K. Ravi and C. Sivakumar for their help during start of the experiment.
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