The association between exposure to aflatoxin, mutation in TP53, infection with hepatitis B virus, and occurrence of liver disease in a selected population in Hyderabad, India

Anitha, S and Raghunadharao, D and Waliyar, F and Sudini, H and Parveen, M and Rao, R and Lava Kumar, P (2014) The association between exposure to aflatoxin, mutation in TP53, infection with hepatitis B virus, and occurrence of liver disease in a selected population in Hyderabad, India. Mutation Research - Genetic Toxicology and Environmental Mutagenesis. pp. 1-20. ISSN 1383-5718

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Abstract

Aflatoxin B1 is a carcinogen produced by Aspergillus flavus and a few related fungi that are often present in many food substances. It interacts synergistically with Hepatitis B or C virus (HBV, HBC) infection, thereby increasing the risk of hepatocellular carcinoma (HCC). The G to T transversion at the third position of codon 249 (AGG) of the TP53 gene, substituting arginine to serine, is the most common aflatoxin-induced mutation linked to HCC. This study examined mutations in TP53 by PCR-RFLP analysis and by measurement of an aflatoxin-albumin adduct as a biomarker for human exposure of aflatoxin B1 by indirect-competitive ELISA, in samples collected from healthy controls as well as patients with hepatitis in Hyderabad, Andhra Pradesh, India. A total of 238 blood samples were analyzed the presence of the G to T mutation. Eighteen of these samples were from HBV-positive subjects, 112 of these were from subjects who had HBV-induced liver cirrhosis, and 108 samples were taken from subjects without HBV infection or liver cirrhosis (control group). The G to T mutation was detected in 10 samples, 8 of which were from subjects positive to both HBV and aflatoxin-albumin adduct in blood (p = 0.07); whilst two were from individuals who were HBV-negative, but positive for the aflatoxin-albumin adduct (p = 0.14). The aflatoxin-albumin adduct was detected in 37 of 238 samples, 29 samples were from HBV-positive subjects and eight were from individuals who were positive for both HBV and the TP53 mutation (p = 0.07). The concentration of aflatoxin-albumin adduct ranged from 2.5 to 667 pg/mg albumin. Despite low incidence of the G to T mutation, its detection in subjects positive to aflatoxin-adducts is indicative of a strong association between the mutation and aflatoxin exposure in India.

Item Type:	Article
Divisions:	RP-Grain Legumes
CRP:	CGIAR Research Program on Agriculture for Nutrition and Health (A4NH)
Uncontrolled Keywords:	Biomarker; TP53 mutation; Aflatoxins; Hepatitis virus; Hepatocellular carcinoma
Subjects:	Others > Genetics and Genomics Others > Food and Nutrition Others > Fertilizer Applications
Depositing User:	Mr Siva Shankar
Date Deposited:	20 Mar 2014 10:13
Last Modified:	21 Nov 2017 08:21
URI:	http://oar.icrisat.org/id/eprint/7651
Official URL:	http://dx.doi.org/doi:10.1016/j.mrgentox.2013.12.0...
Projects:	UNSPECIFIED
Funders:	ICRISAT
Acknowledgement:	We also thank the Statistics Department of ICRISAT for help with the data analysis.
Links:	Google Scholar

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