Isolation and determination of adenosine 3',5'-cyclic monophosphate from plant tissues

    Narayanan, A and Pradet, A (1974) Isolation and determination of adenosine 3',5'-cyclic monophosphate from plant tissues. Indian Journal of Experimental Biology, 12 (3). pp. 287-289. ISSN 0019-5189

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    Abstract

    A procedure has been described to determine picomole levels of adenosine3',5'-cyclic monophosphate (CAMP) in plant materials. The purification of CAMP from other interfering nucleotide and phenolic compounds is made using paper and column chromatography. The resulting CAMP is enzymatically transformed to ATP and estimated by its luminescent reaction with iuciferase. The advantages of this method have been discussed in the light of purification and assay of CAMP. The content of CAMP in lettuce, barley seeds, cabbage leaves, and cultivated mushrooms has been estimated by this procedure

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Others > Plant Pathology
    Depositing User: Mr B K Murthy
    Date Deposited: 30 Jan 2014 01:41
    Last Modified: 30 Jan 2014 01:41
    URI: http://oar.icrisat.org/id/eprint/7403
    Official URL:
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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