Genetic dissection of drought tolerance in chickpea (Cicer arietinum L.)

Varshney, R K and Thudi, M and Nayak, S N and Gaur, P M and Kashiwagi, J and Krishnamurthy, L and Jaganathan, D and Koppolu, J and Bohra, A and Tripathi, S and Rathore, A and Jukanti, A K and Jayalakshmi, V and Anil Kumar, V and Singh, S J and Yasin, Md and Sheshshayee, M S and Viswanatha, K P (2014) Genetic dissection of drought tolerance in chickpea (Cicer arietinum L.). Theoretical and Applied Genetics, 127 (2). pp. 445-462. ISSN 0040-5752

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Abstract

Chickpea (Cicer arietinum L.) is the second most important grain legume cultivated by resource poor farmers in the arid and semi-arid regions of the world. Drought is one of the major constraints leading up to 50 % production losses in chickpea. In order to dissect the complex nature of drought tolerance and to use genomics tools for enhancing yield of chickpea under drought conditions, two mapping populations—ICCRIL03 (ICC 4958 × ICC 1882) and ICCRIL04 (ICC 283 × ICC 8261) segregating for drought tolerance-related root traits were phenotyped for a total of 20 drought component traits in 1–7 seasons at 1–5 locations in India. Individual genetic maps comprising 241 loci and 168 loci for ICCRIL03 and ICCRIL04, respectively, and a consensus genetic map comprising 352 loci were constructed (http://cmap.icrisat.ac.in/cmap/sm/cp/varshney/). Analysis of extensive genotypic and precise phenotypic data revealed 45 robust main-effect QTLs (M-QTLs) explaining up to 58.20 % phenotypic variation and 973 epistatic QTLs (E-QTLs) explaining up to 92.19 % phenotypic variation for several target traits. Nine QTL clusters containing QTLs for several drought tolerance traits have been identified that can be targeted for molecular breeding. Among these clusters, one cluster harboring 48 % robust M-QTLs for 12 traits and explaining about 58.20 % phenotypic variation present on CaLG04 has been referred as “QTL-hotspot”. This genomic region contains seven SSR markers (ICCM0249, NCPGR127, TAA170, NCPGR21, TR11, GA24 and STMS11). Introgression of this region into elite cultivars is expected to enhance drought tolerance in chickpea.

Item Type:	Article
Divisions:	RP-Grain Legumes
CRP:	CGIAR Research Program on Grain Legumes
Subjects:	Mandate crops > Chickpea Others > Genetics and Genomics
Depositing User:	Ms K Syamalamba
Date Deposited:	12 Dec 2013 06:35
Last Modified:	05 Jul 2019 09:16
URI:	http://oar.icrisat.org/id/eprint/7281
Official URL:	http://dx.doi.org/10.1007/s00122-013-2230-6
Projects:	Tropical Legumes I (TLI)
Funders:	Bill and Melinda Gates Foundation-CGIAR Generation Challenge Program
Acknowledgement:	Technical assistance from Srivani Gudipati, Veerender Peddinti and Abdul Gafoor are greatly appreciated. Special thanks are due to Trushar Shah and A. BhanuPrakash for their contribution in uploading the mapping data to CMap server. The work was supported by Tropical Legumes I (TLI) project funded by Bill and Melinda Gates Foundation (BMGF) through CGIAR Generation Challenge Program (GCP; http://www.generationcp.org/). This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium.
Links:	Google Scholar

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