Varshney, R K and Mohan, S M and Gaur, P M and Chamarthi, S K and Singh, V K and Samineni, S and Swapna, N and Sharma, M and Singh, S and Kaur, L and Pande, S (2014) Marker-Assisted Backcrossing to Introgress Resistance to Fusarium Wilt Race 1 and Ascochyta Blight in C 214, an Elite Cultivar of Chickpea. The Plant Genome, 7. pp. 1-11. ISSN 1940-3372
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Abstract
Fusarium wilt (FW) and Ascochyta blight (AB) are two major constraints to chickpea (Cicer arietinum L.) production. Therefore, two parallel marker-assisted backcrossing (MABC) programs by targeting foc1 locus and two quantitative trait loci (QTL) regions, ABQTL-I and ABQTL-II, were undertaken to introgress resistance to FW and AB, respectively, in C 214, an elite cultivar of chickpea. In the case of FW, foreground selection (FGS) was conducted with six markers (TR19, TA194, TAA60, GA16, TA110, and TS82) linked to foc1 in the cross C 214 × WR 315 (FWresistant). On the other hand, eight markers (TA194, TR58, TS82, GA16, SCY17, TA130, TA2, and GAA47) linked with ABQTL-I and ABQTL-II were used in the case of AB by deploying C 214 × ILC 3279 (AB-resistant) cross. Background selection (BGS) in both crosses was employed with evenly distributed 40 (C 214 × WR 315) to 43 (C 214 × ILC 3279) SSR markers in the chickpea genome to select plant(s) with higher recurrent parent genome (RPG) recovery. By using three backcrosses and three rounds of selfing, 22 BC3F4 lines were generated for C 214 × WR 315 cross and 14 MABC lines for C 214 × ILC 3279 cross. Phenotyping of these lines has identified three resistant lines (with 92.7–95.2% RPG) to race 1 of FW, and seven resistant lines (with 81.7–85.40% RPG) to AB that may be tested for yield and other agronomic traits under multilocation trials for possible release and cultivation.
| Item Type: | Article |
|---|---|
| Divisions: | RP-Grain Legumes |
| CRP: | CGIAR Research Program on Grain Legumes |
| Uncontrolled Keywords: | Disease resistance; marker-assisted selection; molecular markers; marker-assisted backcrossing; molecular breeding |
| Subjects: | Mandate crops > Chickpea |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 10 Dec 2013 04:57 |
| Last Modified: | 07 Sep 2017 08:16 |
| URI: | http://oar.icrisat.org/id/eprint/7276 |
| Official URL: | http://dx.doi.org/10.3835/plantgenome2013.10.0035 |
| Projects: | UNSPECIFIED |
| Funders: | Accelerated Crop Improvement Programme (ACIP) from the Department of Biotechnology (DBT), Government of India |
| Acknowledgement: | UNSPECIFIED |
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