Mapping and identification of a Cicer arietinum NSP2 gene involved in nodulation pathway

    Ali, L and Madrid, E and Varshney, R K and Azam, S and Millan, T and Rubio, J and Gill, J (2014) Mapping and identification of a Cicer arietinum NSP2 gene involved in nodulation pathway. Theoretical and Applied Genetics, 127 (2). pp. 481-488. ISSN 0040-5752

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    Abstract

    An intraspecific cross between the mutant non-nodulating genotype PM233, carrying the recessive gene rn1, and the wild-type CA2139 was used to develop two pairs of near-isogenic lines (NILs) for nodulation in chickpea. These pairs of NILs were characterized using sequence tagged microsatellite site (STMS) markers distributed across different linkage groups (LGs) of the chickpea genetic map leading to the detection of polymorphic markers located in LG5. Using this information, together with the genome annotation in Medicago truncatula, a candidate gene (NSP2) known to be involved in nodulation pathway was selected for mapping in chickpea. The full length sequence obtained in chickpea wild-type (CaNSP2) was 1,503 bp. Linkage analysis in an F3 population of 118 plants derived from the cross between the pair of NILS NIL7-2A (nod) × NIL7-2B (non-nod) revealed a co-localization between CaNSP2 and Rn1 gene. These data implicate the CaNSP2 gene as a candidate for identity to Rn1, and suggest that it could act in the nodulation signaling transduction pathway similarly to that in other legumes species.

    Item Type: Article
    Divisions: RP-Grain Legumes
    CRP: CGIAR Research Program on Grain Legumes
    Uncontrolled Keywords: legumes species, Cicer arietinum, Chickpea
    Subjects: Mandate crops > Chickpea
    Others > Genetics and Genomics
    Depositing User: Mr Sanat Kumar Behera
    Date Deposited: 21 Nov 2013 04:59
    Last Modified: 19 Aug 2016 04:40
    URI: http://oar.icrisat.org/id/eprint/7248
    Official URL: http://dx.doi.org/10.1007/s00122-013-2233-3
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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