Naidu, R A and Mayo, M A and Reddy, S V and Jolly, C A and Torrance, L (1997) Diversity among the coat proteins of luteoviruses associated with chickpea stunt disease in India. Annals of Applied Biology, 130 (1). pp. 37-47. ISSN 1744-7348
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Abstract
Chickpea stunt is an important virus disease of chickpea in the Indian subcontinent which is thought to be caused by infection with a luteovirus. Samples of diseased chickpea plants were collected from different chickpea growing regions of India and analysed with a panel of monoclonal antibodies to potato leafroll, beet western yellows and barley yellow dwarf (RPV strain) luteoviruses. The results suggested that more than one luteovirus was present in chickpea crops near ICRISAT Asia Center, Hyderabad. Aphid transmission tests resulted in the separation of two distinct isolates from these samples. One of them (isolate L) was more efficiently transmitted by Myzus persicae than the other (isolate IC). Nucleotide sequence analysis of DNA obtained by reverse transcription-polymerase chain reaction (RT/PCR) amplification revealed that the amino acid sequence of the coat protein of isolate L was 94% identical to that of beet western yellows virus, whereas the coat protein sequence of isolate IC was 82% identical to that of isolate L and 80% identical or less to those of the coat protein of other luteoviruses. Using newly designed “universal luteovirus primers”, a minor sequence variant of isolate IC, which was 96% identical to it in part of the coat protein gene, was detected in the same location during the next season. Only isolate IC could be detected in samples from other locations by either serological or nucleotide sequence analysis
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Chickpea stunt disease; luteovirus; monoclonal antibodies; coat protein; RT-PCR; universal luteovirus PCR primers |
| Subjects: | Mandate crops > Chickpea |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 26 Sep 2013 10:52 |
| Last Modified: | 26 Sep 2013 10:52 |
| URI: | http://oar.icrisat.org/id/eprint/7135 |
| Official URL: | http://dx.doi.org/10.1111/j.1744-7348.1997.tb05781... |
| Projects: | UNSPECIFIED |
| Funders: | Scottish Office Agriculture and Fisheries Department |
| Acknowledgement: | UNSPECIFIED |
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