Varshney, R K and Gaur, P M and Chamarthi, S K and Krishnamurthy, L and Tripathi, S and Kashiwagi, J and Samineni, S and Singh, V K and Thudi, M and Jaganathan, D (2016) Fast-track introgression of “QTL-hotspot” for root traits and other drought tolerance trait in JG 11, an elite and leading variety of chickpea (Cicer arietinum L.). The Plant Genome, 6 (3). pp. 1-9. ISSN 1940-3372
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Abstract
A “QTL-hotspot” containing QTLs for several root and drought tolerance traits was transferred through marker assisted backcrossing (MABC) into JG 11, a leading variety of chickpea in India from the donor parent ICC 4958. Foreground selection with up to 3 SSR (simple sequence repeat) markers namely TAA170, ICCM0249 and STMS11 and background selection with up to 10 AFLP (amplified fragment length polymorphism) primer combinations was undertaken. After undertaking three backcrosses with foreground and background selection and two rounds of selfing, 29 BC3F2 plants homozygous for two markers (ICCM0249 and TAA170) were selected and referred as introgression lines (ILs). Root trait phenotyping of these ILs showed higher rooting depth (RDp, average 115.21±2.24 cm) in all 29 ILs, better root length density (RLD, average 0.41±0.20 cm cm-3) in 25 ILs and higher root dry weight (RDW, average 1.25±0.08 g cyl-1) as compared to the recurrent parent, JG 11 (RDp- 111.70 cm, RLD- 0.39 cm cm-3 and RDW- 1.10 g cyl-1) as well as the donor parent, ICC 4958 (RDp- 114.20 cm, RLD- 0.45 cm cm-3 and RDW- 1.25 g cyl-1). These ILs, developed in 3 years, after multi-location field trials may be released as improved variety with enhanced drought tolerance.
| Item Type: | Article |
|---|---|
| Divisions: | RP-Grain Legumes |
| CRP: | CGIAR Research Program on Grain Legumes |
| Uncontrolled Keywords: | Chickpea; root traits; molecular breeding, marker-assisted selection, drought tolerance |
| Subjects: | Mandate crops > Chickpea Others > Genetics and Genomics |
| Depositing User: | Mr Sanat Kumar Behera |
| Date Deposited: | 25 Aug 2013 09:03 |
| Last Modified: | 14 Apr 2017 05:30 |
| URI: | http://oar.icrisat.org/id/eprint/7066 |
| Official URL: | http://doi.org/10.3835/plantgenome2013.07.0022 |
| Projects: | Tropical Legumes I (TLI- Objective 4: Chickpea) |
| Funders: | Bill and Melinda Gates Foundation |
| Acknowledgement: | This study was undertaken as a part of Tropical Legumes I (TLIObjective 4: Chickpea) funded by Bill & Melinda Gates Foundation (BMGF) through CGIAR Generation Challenge Programme. Thanks are also due to Mohammad Aziz, KVSS Prasad, Vinay Kumar Birapaka, and Nayakoti Swapna for extending their technical help. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. |
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