Murali Krishna, I
(1997)
Comparison of molecular markers for developing pearl millet genetic linkage maps.
Masters thesis, Anna University.
Supervisors
| Supervisors Name | Supervisors ID |
|---|
| Seetharama, N | ICRISAT |
| Kaliraj, P | Anna University |
Abstract
Downy mildew (DM) disease caused by the fungus Sclerosprora
graminicola (Sacc.) Schroet is the major yield reducer of pearl millet cropped in
India and Africa. DM resistance is a complex, quantitatively - inherited trait. The
available RFLP map of this crop developed at the Cambridge laboratory at
Norwich, UK is useful for breeding for DM resistant pearl m~llets by markerassisted
selection and backcrossing. Further, the quantitative trait loci (QTLs)
identified for resistance are also useful for searching for newer sources of
resistance. The present work deals with identification of newer sources and
markers for DM resistance in pearl millet. For this various types of molecular
markers were compared. The marker types being compared includs Isozymes,
RFLP, RAPD, and AFLP.
Among the five accessions of pearl millet selected for the study three
(7042 DMR P2 PI, iP 18298, and lP18292) are downy m~ldew res~stant and the
other two accessions (8438 P5 P2, and 23 D2B ) are downy mildew suseptible.
Out of the eight isozyme systems tried three (peroxidase, esterase, alcohol
dehydrogenase) detected polymorphism between the test entries. RFLP analysis
of these five accessions with the homologous genomic clones (PSM) in
combination with 2 to 4 restriction enzymes (Dral, Hindlll, EcoRI, EcoRV) have
revealed several polymorphic bands. RAPD assays with the same pearl millet
accessions and 16 random primers showed that 50% of the RAPD primers
identified one or more polymorphic bands. AFLP assay of two accessions 843 B
PS P1 and 7042 DMR P2 PI revealed several bands in a 5% denaturing
polyactylamide gel. All the seven primers tested showed polymorphism. AFLP
seem to be technique of choice for further characterization of power of
resistance and genetic linkage mapping.
| Item Type: |
Thesis
(Masters)
|
| Divisions: |
UNSPECIFIED |
| CRP: |
UNSPECIFIED |
| Subjects: |
Mandate crops > Millets |
| Depositing User: |
Users 6 not found. |
| Date Deposited: |
30 Aug 2011 05:28 |
| Last Modified: |
30 Aug 2011 05:29 |
| URI: |
http://oar.icrisat.org/id/eprint/663 |
| Acknowledgement: |
I take this opportunity to express my deep sense of gratitude to my
supervisor, Dr. NSeetharama, senior scientist at ICRISAT for h~s valuable
guidance during my investigation and in writing the thesis.
I express my sincere gratitude to Prof. Kunthala Jayaraman, Dean,
Faculty of Technology, Anna University and Prof. P. Kaliraj, Director, Centre For
Biotechnology for allowing me to work on this project.
I am also grateful to Dr. Manjula Sritharan and Dr. R.B.Narayanan for
their help in getting me this project.
I like to express my gratefulness to Dr. Diwakar, Program Leader, TAFP
and to Mr. K.D.V Prasad, Design and Development Division, ICRISAT for the~r
help in carrying out my work at ICRISAT.
I wish to express my heartfelt gratitude and indebtedness to Leela,
Research Associate, and Mr. Yosuf, CMBD, ICRISAT without whose help th~s
work might not be in its present form.
I wish to specially thank Shyamala, Dr. Sairam, Dr. Hiren, Srinivas, Anand
and Sravan for their help and valuable suggestions.
I specially thank Hanumantha Rao, Prashanth, Laxminarayana, Malla
Reddy, and others in CMBD lab.
I also thank my friends Erik, Raj, Leela Prasad and other classmates for their help.
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