Swathi, M and Mohanraj, S S and Srinivas, C and Prasad, E R and Venkatrao, V and Mallikarjuna, N and Dutta-Gupta, A and Padmasree, K (2012) Cajanus cajan (cv. C11): An Important Non-Host Resource for Proteinase Inhibitors Active against Larval Midgut Proteinases of Lepidopteran Insect Pest Achaea janata. Functional Plant Science and Biotechnology, 6 (1). pp. 82-85. ISSN 1749-0472
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Abstract
Our previous studies indicated that seeds of various cultivars and wild relatives of pigeonpea possessed proteinase inhibitors (PIs) which were active against midgut trypsin-like proteinases of lepidopteran insects. In the present study a detailed analysis of PIs distribution in mature seeds of five different cultivars of Cajanus cajan and thirteen different accessions of Cajanus platycarpus was carried out. Further the inhibitory potential of these PIs against larval midgut trypsin-like proteinases of Achaea janata was also evaluated. Among the chosen cultivars and C. platycarpus accessions, the cultivar ‘ICP 7118’ also called as ‘C11’ showed highest inhibitory potential against A. janata larval midgut trypsin-like proteinases (AJPs). The inhibitory potential of PIs from ‘C11’ was 25 and 106 fold higher against AJPs, compared to bovine pancreatic trypsin and chymotrypsin, respectively. Further, the activity profile of PIs of ‘C11’ on gelatin-polyacrylamide gel demonstrated the presence of higher number of trypsin inhibitor isoforms as well as stronger inhibitory potential against AJPs, when compared with C. platycarpus accessions. Thus, the results from the present investigation confirmed that the cultivar ‘C11’ of C. cajan is the best non-host resource for PIs active against AJPs and these PIs can be readily exploited further as ecofriendly pesticide in the management of this non-host lepidopteran insect pest A. janata using transgenic technology.
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Achaea janata, gelatin-PAGE, gut proteinases, pigeonpea, proteinsae inhibitor |
| Subjects: | Mandate crops > Pigeonpea Others > Entomology |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 15 Feb 2013 17:24 |
| Last Modified: | 14 Sep 2013 13:57 |
| URI: | http://oar.icrisat.org/id/eprint/6587 |
| Official URL: | http://www.globalsciencebooks.info/JournalsSup/12F... |
| Projects: | UNSPECIFIED |
| Funders: | CSIR |
| Acknowledgement: | UNSPECIFIED |
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