Linkage mapping and QTL analysis of drought related traits in Groundnut (Arachis hypogaea L.)

Gautami, B (2012) Linkage mapping and QTL analysis of drought related traits in Groundnut (Arachis hypogaea L.). PHD thesis, International Crops Research Institute for the Semi-Arid Tropics.

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Supervisors

Supervisors NameSupervisors ID
Hoisington, D AICRISAT
Narasu, M LJNTUH

Abstract

Groundnut (Arachis hypogaea L.), commonly called peanut, is one of the most important oilseed crops in the smallholder-farming sectors of the semi-arid tropical regions of the world where drought is the major production constraint. Until recently, the low level of molecular diversity in the cultivated groundnut genome and the scarcity of co-dominant DNA-based molecular markers were critical constraints in using modern genomics in groundnut improvement. To increase the number of molecular markers for groundnut, 23 novel simple sequence repeat (SSR or micro-satellite) markers were isolated from a SSR-enriched genomic library. These new markers, along with 3215 already available markers from different sources were tested for detecting polymorphism among parental genotypes of the two recombinant inbred line (RIL) mapping populations (ICGS 76 × CSMG 84-1 and ICGS 44 × ICGS 76) to understand the genetic basis and identification of QTLs for drought related traits. As a result, two new genetic linkage maps were developed with 119 (2208 cM) and 82 (831 cM) marker loci. In addition, a consensus map consisting of 293 SSR loci located across 20 linkage groups and spanning a map distance of 2841 cM was constructed using the two new genetic maps (from the present study) and the reference map TAG 24 × ICGV 86031....

Item Type: Thesis (PHD)
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Subjects: Mandate crops > Groundnut
Others > Genetics and Genomics
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 29 Jan 2013 05:44
Last Modified: 20 Nov 2015 05:06
URI: http://oar.icrisat.org/id/eprint/6479
Acknowledgement: With great pleasure I express my esteemed gratitude and deep sense of indebtedness to my respectful supervisor Dr. Dave Hoisington, the Deputy Director General (DDG)-Research, ICRISAT for his invaluable guidance, critical evaluation, emending suggestions and constant encouragement throughout to bring out this thesis successful. I feel it to be a privilege to work under his able guidance. I wish to record my profound gratitude and sincere thanks to the personification of generosity and kindness to my co-supervisor Prof. M. Laxmi Narasu, Centre for Biotechnology, JNTUH University for her expedient advice, ever-encouraging suggestions, timely help, invaluable support, kind concern and consideration regarding my academic requirements during this tenure of research work. This dissertation would not have been possible without the guidance and the kind help of Dr. Rajeev K Varshney, Principal Scientist-Applied Genomics, ICRISAT and Leader Theme1-Comparative and Applied Genomics (CAG), Generation Challenge Programme. I am sincerely obliged and indebted for his peer guidance, constructive comments, enthusiastic discussions, endless support, encouragement and able direction throughout my research project. I wish to express my sincere thanks for the critical evaluation and emending suggestions in the preparation of scientific papers for journals and in achieving the final form of dissertation. I wish to express my thanks and gratitude to Dr. William D. Dar, the Director General, ICRISAT for giving me an opportunity to work in this esteemed organization
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