Methods for the Estimation of Protein in Pigeonpea [Cajanus cajan (L.) Millsp.] and the Relationship Between Whole Grain and Dhal Protein Contents

    Singh, U and Jambunathan, R (1981) Methods for the Estimation of Protein in Pigeonpea [Cajanus cajan (L.) Millsp.] and the Relationship Between Whole Grain and Dhal Protein Contents. Journal of the Science of Food and Agriculture, 32 (7). pp. 705-710. ISSN 1097-0010

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    Abstract

    Proteindeterrninations of 172 grain and dhal (decorticated dry split seed) pigeonpea samples were carried out using three methods: (a) rnicrow Kjeldahl (MKJ), (b) colorimetric estimation of NH4+ with phenol hypochlorite reagents qsing the Technicon Auto-Analyser (TAA), and (c) dye-binding capacity (DBC) method using Acid Orange 12 dye: Protein perceIltages determined by the TAA and MKJ methods were highly correlated for whole-grain (0'948**) and dhal (0·967**) samples. The DBC method gave reliable results for dhal samples only. In the DBC procedure, higher protein percentages were recorded with smaller flour par'tides and longer mixing time, but different temperatures and durations of heating had no effect. Positive and highly significant correlations were obtained between the protein values of whole-grain and dhal samples in all the methods. Small irains gave a lower correlation between whole-grain and dhal protein content due to the observed negative c;:orrelation between grain size and percentage of seed~coat.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Uncontrolled Keywords: Food Proteins, Dhal
    Subjects: Mandate crops > Pigeonpea
    Others > Food and Nutrition
    Depositing User: Mr Siva Shankar
    Date Deposited: 15 Nov 2012 04:40
    Last Modified: 15 Nov 2012 04:40
    URI: http://oar.icrisat.org/id/eprint/6216
    Official URL: http://dx.doi.org/10.1002/jsfa.2740320711
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: Technical assistance ofN. S. Rao and G. L. Waghray is gratefully acknowledged and K, B. Saxena for supplying the seed material.
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