Investigation of recent population bottlenecks in Kenyan wild sorghum populations (Sorghum bicolor (L.) Moench ssp. verticilliflorum (Steud.) De Wet) based on microsatellite diversity and genetic disequilibria

    Muraya, M M and Sagnard, F and Parzies, H K (2010) Investigation of recent population bottlenecks in Kenyan wild sorghum populations (Sorghum bicolor (L.) Moench ssp. verticilliflorum (Steud.) De Wet) based on microsatellite diversity and genetic disequilibria. Genetic Resources and Crop Evolution, 57 (7). pp. 995-1005. ISSN 1573-5109

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    Abstract

    Identifying populations that have recently suffered a severe reduction in size is particularly important for their conservation as they are likely to suffer an increased risk of genetic erosion. We investigated the presence of recent bottlenecks in two wild sorghum populations from different eco-geographical conditions in Kenya employing 18 microsatellite markers. Microsatellite analysis showed high allelic diversity in the two populations, with a mean of 4.11 and 6.94 alleles per locus in the North-West wild sorghum population (NWWSP) and the South-East wild sorghum population (SEWSP), respectively. The mean observed heterozygosity was 0.34 and 0.56 in NWWSP and SEWSP, respectively. A large long-term effective populations size for both populations was observed assuming either an infinite allele model or a stepwise mutation model. There was no apparent loss of genetic variability for either of the populations. Test of heterozygosity excess indicated that a recent bottleneck in the two populations is highly unlikely. Furthermore, analysis of the allele frequency distribution revealed an L-shaped distribution which would not have been observed in case a recent bottleneck had reduced genetic variability in the two populations. The fact that most loci displayed a significant heterozygosity deficiency could be explained by population subdivision and the mixed mating system exhibited by wild sorghum populations. Furthermore, the possibility of a historical expansion of wild sorghum populations and presence of null alleles could not be ruled out.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Uncontrolled Keywords: Bottleneck – Genetic diversity – Linkage disequilibrium – Microsatellite markers – Null allele – Sorghum bicolor ssp. verticilliflorum – Wild sorghum population
    Subjects: Mandate crops > Sorghum
    Others > Genetics and Genomics
    Others > Agriculture-Farming, Production, Technology, Economics
    Depositing User: Mr Sanat Kumar Behera
    Date Deposited: 02 Oct 2012 13:40
    Last Modified: 02 Oct 2012 13:40
    URI: http://oar.icrisat.org/id/eprint/6160
    Official URL: http://dx.doi.org/10.1007/s10722-010-9539-7
    Projects: UNSPECIFIED
    Funders: United States Agency for International Development, Biotechnology and Biodiversity Interface Program, Germany Academic Exchange Service
    Acknowledgement: This study was funded by the United States Agency for International Development (USAID) Biotechnology and Biodiversity Interface Program (BBI), the Institute of Plant Breeding and Population Genetics at the University of Hohenheim, Germany, and Germany Academic Exchange Service (DAAD: A0523923). We are grateful to Kenya Agricultural Research Institute and Ben Kanyenji who supervised the collection of genetic materials in full compliance with regulations according to the Convention on Biological Diversity (CBD).
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