Identification of unique alleles and assessment of genetic diversity of rabi sorghum accessions using simple sequence repeat markers

Thudi, M and Fakrudin, B (2011) Identification of unique alleles and assessment of genetic diversity of rabi sorghum accessions using simple sequence repeat markers. Journal of Plant Biochemistry and Biotechnology, 20 (1). pp. 74-83. ISSN 0971-7811

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Abstract

Genetic diversity among 42 sorghum accessions representing landraces (19), advanced breeding lines (16), local cultivars (2) and release varieties (5) with 30 simple sequence repeat (SSR) markers revealed 7.6 mean number of alleles per locus showing 93.3% polymorphism and an average polymorphism information content of 0.78 which range from 0.22 (Xtxp12) and 0.91(Xtxp321). The average heterozygosity and effective number of alleles per locus were 0.8 and 6.65 respectively. Cluster analysis based on microsatellite allelic diversity clearly demarcated the accessions into ten clusters. A total of 24 unique alleles were obtained from seven SSR loci in 23 accessions in a size range of 110–380 bp; these unique alleles may serve as diagnostic tools for particular region of the genome of respective genotypes. Selected SSR markers from different linkage groups provided an accurate way of determining genetic diversity at the molecular level

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Sorghum
Depositing User:	Ms K Syamalamba
Date Deposited:	05 Jun 2012 09:05
Last Modified:	05 Jun 2012 09:05
URI:	http://oar.icrisat.org/id/eprint/5949
Official URL:	http://dx.doi.org/10.1007/s13562-010-0028-z
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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