Billot, C and Rivallan, R and Sall, M N and Fonceka, D and Deu, M and Glaszmann, J C and Noyer, J L and Rami, J F and Risterucci, A M and Wincker, P and Ramu, P and Hash, C T (2012) A reference microsatellite kit to assess for genetic diversity of Sorghum bicolor (Poaceae). American Journal of Botany, 99 (6). e245-e250. ISSN 1537-2197
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Abstract
Premise of the study: Discrepancies in terms of genotyping data are frequently observed when comparing simple sequence repeat (SSR) data sets across genotyping technologies and laboratories. This technical concern introduces biases that hamper any synthetic studies or comparison of genetic diversity between collections. To prevent this for Sorghum bicolor, we developed a control kit of 48 SSR markers. • Methods and Results: One hundred seventeen markers were selected along the genome to provide coverage across the length of all 10 sorghum linkage groups. They were tested for polymorphism and reproducibility across two laboratories (Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement [CIRAD], France, and International Crops Research Institute for the Semi-Arid Tropics [ICRISAT], India) using two commonly used genotyping technologies (polyacrylamide gel–based technology with LI-COR sequencing machines and capillary systems with ABI sequencing apparatus) with DNA samples from a diverse set of 48 S. bicolor accessions. • Conclusions: A kit for diversity analysis (http://sat.cirad.fr/sat/sorghum_SSR_kit/) was developed. It contains information on 48 technically robust sorghum microsatellite markers and 10 DNA controls. It can further be used to calibrate sorghum SSR genotyping data acquired with different technologies and compare those to genetic diversity references
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Diversity kit; Poaceae; Sorghum bicolor; standardization; simple sequence repeat markers |
| Subjects: | Mandate crops > Sorghum Others > Genetics and Genomics |
| Depositing User: | Mr Sanat Kumar Behera |
| Date Deposited: | 02 Jun 2012 08:28 |
| Last Modified: | 10 Jun 2014 03:53 |
| URI: | http://oar.icrisat.org/id/eprint/5946 |
| Official URL: | http://dx.doi.org/10.3732/ajb.1100548 |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
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