Tissue culture response of CIMMYT elite bread wheat cultlvars and evaluation of regenerated plants

Bohorova, N E and Ginkel, M van and Rajaram, V and Hoisington, D A (1995) Tissue culture response of CIMMYT elite bread wheat cultlvars and evaluation of regenerated plants. Cereal Research Communications, 23 (3). pp. 243-249. ISSN 1788-9170

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Abstract

Six elite CIMMYT bread wheat genotypes Pavon 76, Seri M82, Opata M85, Mochis T88, Baviacora M93, and the advanced line Attila were evaluated for their response to in vitro tissue culture. Donor plants were grown at EI Batan and CIANO experiment stations in Mexico in 1992. Immature embryos, about 1.0 mm in length, were excised and placed scutellum side up on callus initiation media. Basal N6 medium supplemented with dicamba (E1), MS medium containing 2,40 (E3), or MS medium containing 2,40 plus different amino acids (E5) was used for callus initiation and maintenance. Plant regeneration and rooting were achieved on basal MS medium with IAA and BAP. Embryogenic calli with regeneration potentials of 10-92% were obtained on E3 and E5 media; each embryo produced 5-50 plants. A total of 800 regenerated plants were transferred to pots in the greenhouse for evaluation and seed production. Of the regenerated plants, 85% were morphologically normal. reached full maturity, and produced seeds. Seeds (R1) of 360 plants regenerated from Mochis T88, Baviacora M93, and Altila were planted in the field. Field observations indicated that embryos cultured on E3 medium resulted in 29% of the progeny producing deviating plants, compared with 21 % of those grown on E5 medium. These results will form the basis for future efforts aimed at transforming CIMMYT bread wheat varieties. IntroductionSix elite CIMMYT bread wheat genotypes Pavon 76, Seri M82, Opata M85, Mochis T88, Baviacora M93, and the advanced line Attila were evaluated for their response to in vitro tissue culture. Donor plants were grown at EI Batan and CIANO experiment stations in Mexico in 1992. Immature embryos, about 1.0 mm in length, were excised and placed scutellum side up on callus initiation media. Basal N6 medium supplemented with dicamba (E1), MS medium containing 2,40 (E3), or MS medium containing 2,40 plus different amino acids (E5) was used for callus initiation and maintenance. Plant regeneration and rooting were achieved on basal MS medium with IAA and BAP. Embryogenic calli with regeneration potentials of 10-92% were obtained on E3 and E5 media; each embryo produced 5-50 plants. A total of 800 regenerated plants were transferred to pots in the greenhouse for evaluation and seed production. Of the regenerated plants, 85% were morphologically normal. reached full maturity, and produced seeds. Seeds (R1) of 360 plants regenerated from Mochis T88, Baviacora M93, and Altila were planted in the field. Field observations indicated that embryos cultured on E3 medium resulted in 29% of the progeny producing deviating plants, compared with 21 % of those grown on E5 medium. These results will form the basis for future efforts aimed at transforming CIMMYT bread wheat varieties. Introduction

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	culture media, deviating plants, embryogenic callus, regeneration, Triticum aestivum
Subjects:	Others > Wheat
Depositing User:	Mr Siva Shankar
Date Deposited:	19 Mar 2012 06:06
Last Modified:	19 Mar 2012 06:06
URI:	http://oar.icrisat.org/id/eprint/5756
Official URL:
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	[NOTE: D. A. Hoisington is presently with ICRISAT (Patancheru)]---The authors would like to thank Baclirsa Luna and LetiCia Dlaz for therr excellent technical assistance They also thank Alma McNab for helpmg to prepare thiS manuscript, Sarah Fennell and Etienne Duveilier for therr useful comments, and J Crossa for statistical analYSIS
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