Methylparathion- and carbofuran-induced mitochondrial dysfunction and oxidative stress in Helicoverpa armigera (Noctuidae: Lepidoptera)

Akbar, S Md. and Sharma, H C and Jayalakshmi, S K and Sreeramulu, K (2012) Methylparathion- and carbofuran-induced mitochondrial dysfunction and oxidative stress in Helicoverpa armigera (Noctuidae: Lepidoptera). Pesticide Biochemistry and Physiology, 103 (1). pp. 31-37. ISSN 1095-9939

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Abstract

The cotton bollworm, Helicoverpa armigera is a polyphagous pest of several crops in Asia, Africa, and the Mediterranean Europe. Organophosphate and carbamate insecticides are used on a large-scale to control Helicoverpa. Therefore, we studied the effect of methylparathion and carbofuran, an organophosphate and carbamate insecticide, respectively, on oxidative phosphorylation and oxidative stress in H. armigera larvae to gain an understanding of the different target sites of these insecticides. It was observed that state III and state IV respiration, respiratory control index (RCI), and P/O ratios were inhibited in a dose-dependent manner by methylparathion and carbofuran under invitro and in vivo conditions. Methylparathion and carbofuran inhibited complex II by ∼ 45% and 30%, respectively. Lipid peroxidation, H2O2 content, and lactate dehydrogenase (LDH) activity increased and glutathione reductase (GR) activity decreased in a time- and dose-dependent manner in insecticide-fed larvae. However, catalase activity was not affected in insecticide-fed larvae. Larval growth decreased by ∼ 64 and 67% in larvae fed on diets with sub-lethal doses of methylparathion and carbofuran. The results suggested that both the insecticides impede the mitochondrial respiratory functions and induced lipid peroxidation, H2O2, and LDH leak, leading to oxidative stress in cells, which contribute to deleterious effects of these insecticides on the growth of H. armigera larvae, along with their neurotoxic effects.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Helicoverpa armigera; Mitochondria; Respiration; Oxidative stress; Methylparathion; Carbofuran
Subjects:	Mandate crops > Chickpea Mandate crops > Pigeonpea Mandate crops > Groundnut Mandate crops > Sorghum Others > Entomology
Depositing User:	Mr Siva Shankar
Date Deposited:	09 Mar 2012 03:50
Last Modified:	18 Mar 2014 05:47
URI:	http://oar.icrisat.org/id/eprint/5693
Official URL:	http://dx.doi.org/10.1016/j.pestbp.2012.02.005
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	We thank Mr V Venkateshwar Rao and Mr Chitti B Giddi, for providing the insect culture and help with the experiments. We also thank UGC, for providing JRF under RFSMS scheme to SMD Akbar, UGC – SAP (DSR - I), New Delhi, to KS and the Ministry of Agriculture, Government of India, to HCS.
Links:	Author

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