Hossain, Z A K M and et al, . (2008) Detection, isolation and characterization of a root-exuded compound, methyl 3-(4-hydroxyphenyl) propionate, responsible for biological nitrification inhibition by sorghum (Sorghum bicolor). New Phytologist, 180 (2). pp. 442-451. ISSN 1469-8137
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Abstract
Nitrification results in poor nitrogen (N) recovery and negative environmental impacts in most agricultural systems. Some plant species release secondary metabolites from their roots that inhibit nitrification, a phenomenon known as biological nitrification inhibition (BNI). Here, we attempt to characterize BNI in sorghum (Sorghum bicolor).• In solution culture, the effect of N nutrition and plant age was studied on BNI activity from roots. A bioluminescence assay using recombinant Nitrosomonas europaea was employed to determine the inhibitory effect of root exudates. One major active constituent was isolated by activity-guided HPLC fractionations. The structure was analysed using NMR and mass spectrometry. Properties and the 70% inhibitory concentration (IC70) of this compound were determined by in vitro assay.• Sorghum had significant BNI capacity, releasing 20 allylthiourea units (ATU) g−1 root DW d−1. Release of BNI compounds increased with growth stage and concentration of supply. -grown plants released several-fold higher BNI compounds than -grown plants. The active constituent was identified as methyl 3-(4-hydroxyphenyl) propionate.• BNI compound release from roots is a physiologically active process, stimulated by the presence of . Methyl 3-(4-hydroxyphenyl) propionate is the first compound purified from the root exudates of any species; this is an important step towards better understanding BNI in sorghum.
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | biological nitrification inhibition;BNI compounds;methyl 3-(4-hydroxyphenyl) propionate;sorghum (Sorghum bicolor) |
| Subjects: | Mandate crops > Sorghum Others > Soil Science |
| Depositing User: | Mr Sanat Kumar Behera |
| Date Deposited: | 19 Feb 2012 08:27 |
| Last Modified: | 19 Feb 2012 08:27 |
| URI: | http://oar.icrisat.org/id/eprint/5544 |
| Official URL: | http://dx.doi.org/10.1111/j.1469-8137.2008.02576.x |
| Projects: | UNSPECIFIED |
| Funders: | Goverment of Japan- Ministry of Agriculture, Forestry and Fisheries |
| Acknowledgement: | We thank Dr Matthias Wissuwa, Takeshi Watanabe and Dr Juan Pariasca Tanaka for their valuable suggestions during this research. We also thank Ms Ikuko Maeda for technical assistance in NMR analysis. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Agriculture, Forestry and Fisheries of Japan. |
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