Nitrification Inhibitors from the Root Tissues of Brachiaria humidicola, a Tropical Grass

Gopalakrishnan, S and et al, . (2007) Nitrification Inhibitors from the Root Tissues of Brachiaria humidicola, a Tropical Grass. Journal of Agricultural and Food Chemistry, 55 (4). pp. 1385-1388. ISSN 1520-5118

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Abstract

Nitrification inhibitory activity was found in root tissue extracts of Brachiaria humidicola, a tropical pasture grass. Two active inhibitory compounds were isolated by activity-guided fractionation, using recombinant Nitrosomonas europaea containing luxAB genes derived from the bioluminescent marine gram-negative bacterium Vibrio harveyi. The compounds were identified as methyl-p-coumarate and methyl ferulate, respectively. Their nitrification inhibitory properties were confirmed in chemically synthesized preparations of each. The IC50 values of chemically synthesized preparations were 19.5 and 4.4 μM, respectively. The ethyl, propyl, and butyl esters of p-coumaric and ferulic acids inhibited nitrification, whereas the free acid forms did not show inhibitory activity.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Brachiaria humidicola; nitrification inhibitor; methyl ferulate; methyl-p-coumarate
Subjects:	Others > Soil Science Others > Agriculture-Farming, Production, Technology, Economics
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	18 Feb 2012 11:09
Last Modified:	18 Feb 2012 11:09
URI:	http://oar.icrisat.org/id/eprint/5543
Official URL:	http://pubs.acs.org/doi/abs/10.1021/jf062593o
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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