Genetic diversity and pathogenic variability among Indian isolates of Fusarium udum infecting pigeonpea (Cajanus cajan (L.) millsp.)

    Mesapogu, S and Bakshi, A and Babu, B K and Reddy, S S and Saxena, S and Arora, D K (2012) Genetic diversity and pathogenic variability among Indian isolates of Fusarium udum infecting pigeonpea (Cajanus cajan (L.) millsp.). International Research Journal of Agricultural Science and Soil Science, 2 (1). pp. 51-57. ISSN 2251-0044

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    Abstract

    Genetic diversity and pathogenic variability among Fusarium udum isolates collected from different geographical locations of India were studied. All the isolates exhibited variable levels of virulence against a susceptible pigeonpea cultivar (T-21). The genetic diversity allelic variations among these isolates were estimated using RAPD molecular markers. All the thirteen RAPD primers were found to be highly reproducible and produced a total of 126 loci of which 69 loci were polymorphic. Primer OPB 17 amplified highest number of polymorphic bands with maximum polymorphic information content (PIC) of 4.00. Percentage of polymorphism revealed by individual primers varied from 33.3 to 76.9% with an average of 53.4%. Further, cluster analysis of OPB-17 and provided a substantially discrimination of all the isolates. Our results showed a high degree of variability in pathogenicity and genetic diversity among the populations. Therefore the present studies indicate that the F. udum may have significant impact towards the emergence/evolutionary development.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Uncontrolled Keywords: Fusarium wilt, pathogenecity, pigeonpea, RAPD
    Subjects: Mandate crops > Pigeonpea
    Depositing User: Users 6 not found.
    Date Deposited: 31 Jan 2012 05:12
    Last Modified: 31 Jan 2012 05:12
    URI: http://oar.icrisat.org/id/eprint/5388
    Official URL: http://interesjournals.org/IRJAS/Pdf/2012/January/...
    Projects: UNSPECIFIED
    Funders: Indian Council of Agricultural Research
    Acknowledgement: We thank Indian Council of Agricultural Research, New Delhi, India, for providing financial assistance under the Application of Microbes in Agriculture and Allied Sectors (AMAAS) project.
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