Upadhyaya, H D and Pundir, R P S and Singh, S and Gowda, C L L (2006) Procedures and protocols to maintain purity and viability of peanut (groundnut) germplasm. In: Improving yield and economic viability of peanut production in Papua New Guinea and Australia: proceedings of a workshop, 18–19 October 2005, Lae, Papua New Guinea.
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Abstract
Successful genebank operations should ensure that pure and healthy seeds are maintained for utilisation in crop improvement and conserving for posterity. Maintaining peanut germplasm in the genebank requires considerable attention. Some protocols followed to maintain purity and viability of peanut germplasm are outlined in this paper. Some distinct measures required for maintenance of peanut germplasm are: (i) raising the peanut crop in well drained light soils, (ii) growing a minimum of 160 plants per accession to retain genetic integrity, (iii) verifi cation of accessions’ identity at various stages of crop growth to remove off-type and diseased plants, (iv) harvesting crop at optimum maturity to obtain healthy seeds, (v) avoiding exposure of the freshly harvested pods to strong sunlight (temperature >40°C) to maintain seed viability in storage, (vi) avoiding damage while picking and shelling of pods manually, (vii) conserving seeds at appropriate moisture content, in medium term (4°C; 30% RH) at 7% and in long term (-20°C) at 3–5% and (viii) monitoring of seed stock, viability and health at regular intervals to assess the need for regeneration. As part of the Australian Centre for International Agricultural Research (ACIAR) project ASEM 2001/055, ICRISAT supplied 46 peanut (groundnut) breeding lines for evaluation in Papua New Guinea (PNG) environments to identify varieties with superior yield and quality over the local check which could be introduced for cultivation in PNG. It is critical that the PNG organisations take responsibility for maintaining genetic purity of the newly introduced peanut germplasm. This paper describes the procedures and protocols that are followed at ICRISAT for maintaining the genetic purity of peanut germplasm. These lines can be maintained by following the procedures described in this paper.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Mandate crops > Groundnut |
| Depositing User: | Library ICRISAT |
| Date Deposited: | 02 Jan 2012 13:04 |
| Last Modified: | 02 Jan 2012 13:04 |
| URI: | http://oar.icrisat.org/id/eprint/5193 |
| Acknowledgement: | UNSPECIFIED |
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