Pseudomonas aeruginosa inhibits the plant cell wall degrading enzymes of Sclerotium rolfsii and reduces the severity of groundnut stem rot

Kishore, G K and Pande, S and Rao, J N and Podile, A R (2005) Pseudomonas aeruginosa inhibits the plant cell wall degrading enzymes of Sclerotium rolfsii and reduces the severity of groundnut stem rot. European Journal of Plant Pathology, 113 (3). pp. 315-320. ISSN 0929-1873

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Abstract

Three hundred and ninety-three groundnut-associated bacterial strains, applied both as seed treatment and soil amendment, were evaluated for control of stem rot disease (caused by Sclerotium rolfsii) of groundnut in a controlled environment. Twelve strains significantly (P=0.01) reduced the incidence of stem, rot of which groundnut seed endophytes Pseudomonas aeruginosa GSE 18 and GSE 19 reduced the seedling mortality by 54% and 58%, compared to the control. In dual cultures, the 12 biocontrol strains reduced the mycelial growth of S. rolfsii by 32%-74% as compared to the control. Cell-free culture filtrates of P. aeruginosa GSE 18 and GSE 19 inhibited the activity in vitro of the cell wall-degrading enzymes (CWDE) polygalacturonase and cellulase by S. rolfsii up to a maximum of 55% and 50%, respectively, when measured 6 days after inoculation. Pseudomonas aeruginosa GSE 18 and GSE 19 with a known tolerance to thiram, a commonly used seed dressing fungicide, suppressed the growth of S. rolfsii, inhibited the activity of CWDE, and reduced the incidence of stem rot, suggesting the usefulness of these biocontrol strains as components in the integrated management of groundnut stem rot.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Groundnut
Depositing User:	Library ICRISAT
Date Deposited:	11 Dec 2011 09:24
Last Modified:	11 Dec 2011 09:24
URI:	http://oar.icrisat.org/id/eprint/4864
Official URL:	http://dx.doi.org/10.1007/s10658-005-0295-z
Projects:	UNSPECIFIED
Funders:	AP-Netherlands Biotechnology Programme
Acknowledgement:	Financial support to ARP by the AP-Netherlands Biotechnology Programme, Institute of Public Enterprise, Hyderabad and special assistance programme of University Grants Commission, New Delhi, and SP by the Department for International Development, UK are gratefully acknowledged.
Links:	Author

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