Mapping and validation of QTLs for resistance to an Indian isolate of Ascochyta blight pathogen in chickpea

Kottapalli, P and Gaur, P M and Katiyar, S K and Crouch, J H and Buhariwalla, H K and Pande, S and Gali, K K (2009) Mapping and validation of QTLs for resistance to an Indian isolate of Ascochyta blight pathogen in chickpea. Euphytica, 165 (1). pp. 79-88.

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Abstract

Ascochyta blight (AB) caused by Ascochyta rabiei, is globally the most important foliar disease that limits the productivity of chickpea (Cicer arietinum L.). An intraspecific linkage map of cultivated chickpea was constructed using an F2 population derived from a cross between an AB susceptible parent ICC 4991 (Pb 7) and an AB resistant parent ICCV 04516. The resultant map consisted of 82 simple sequence repeat (SSR) markers and 2 expressed sequence tag (EST) markers covering 10 linkage groups, spanning a distance of 724.4 cM with an average marker density of 1 marker per 8.6 cM. Three quantitative trait loci (QTLs) were identified that contributed to resistance to an Indian isolate of AB, based on the seedling and adult plant reaction. QTL1 was mapped to LG3 linked to marker TR58 and explained 18.6% of the phenotypic variance (R2) for AB resistance at the adult plant stage. QTL2 and QTL3 were both mapped to LG4 close to four SSR markers and accounted for 7.7% and 9.3%, respectively, of the total phenotypic variance for AB resistance at seedling stage. The SSR markers which flanked the AB QTLs were validated in a half-sib population derived from the same resistant parent ICCV 04516. Markers TA146 and TR20, linked to QTL2 were shown to be significantly associated with AB resistance at the seedling stage in this half-sib population. The markers linked to these QTLs can be utilized in marker-assisted breeding for AB resistance in chickpea.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Ascochyta blight; Chickpea; Cicer arietinum; Genetic linkage map; QTL mapping; SSR markers
Subjects:	Mandate crops > Chickpea
Depositing User:	Library ICRISAT
Date Deposited:	26 Aug 2011 04:43
Last Modified:	26 Aug 2011 04:44
URI:	http://oar.icrisat.org/id/eprint/387
Official URL:	http://dx.doi.org/10.1007/s10681-008-9762-x
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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