Detection of a mycoplasma-like organism in peanut plants with witches' broom using indirect enzyme-linked immunosorbent assay (ELISA)

Hobbs, H A and Reddy, D V R and Reddy, A S (1987) Detection of a mycoplasma-like organism in peanut plants with witches' broom using indirect enzyme-linked immunosorbent assay (ELISA). Plant Pathology, 36 (2). pp. 164-167. ISSN 00320862

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Abstract

The mycoplasma-like organism (MLO) associated with peanut (groundnut) witches’ broom (PWB) from India was partially purified and an antiserum produced against it. Using a protein A indirect enzyme-linked immunosorbent assay (ELISA) procedure, PWB MLO was detected in crude extracts of leaves. stems and pegs of infected peanut plants, although stems were a better source than leaves and pegs. Extracts of infected tissues of three diseases of assumed MLO etiology in India, little leaf of brinjal (eggplant). I med rosed witches “broom, and Daturd sp. witches’ broom, failed to react with the PWB MLO antiserum.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Groundnut
Depositing User:	Mr Charan Sai Ch
Date Deposited:	03 Nov 2011 03:25
Last Modified:	12 Apr 2012 04:59
URI:	http://oar.icrisat.org/id/eprint/3595
Official URL:	http://dx.doi.org/10.1111/j.1365-3059.1987.tb02216...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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