Peanut (Arachis hypogaea L.)

Sharma, K K and Bhatnagar-Mathur, P (2006) Peanut (Arachis hypogaea L.). In: Methods in Molecular Biology: Agrobacterium Protocols. Springer, Totowa, New Jersey, pp. 347-358. ISBN 1-58829-536-2

PDF - Published Version Restricted to ICRISAT users only Download (181kB) | Request a copy

Abstract

Arachis hypogea (peanut, groundnut), an annual oil seed belonging to the Leguminosae family and the Papillionacea subfamily, is a legume native to South America but now grown in diverse environments in six continents between latitudes 40°N and 40°S. Arachis hypogea can grow in a wide range of climatic conditions. The low yields of this crop are mainly attributed to unreliable rainfall patterns with frequent droughts, lack of highyielding adapted cultivars, damage by diseases and pests, poor agronomic practices, and limited use of inputs. Genetic engineering approaches have been shown to be comparatively fast, leading to better isolation and cloning of desired traits for combating the various biotic and abiotic stresses. This chapter describes an Agrobacterium-mediated transformation protocol in peanut using the cotyledon system. The system described here is potentially applicable to a vast range of genotypes with a high transformation frequency of >70% based on the preliminary molecular data, indicating the production of a large number of independently transformed transgenic plants. The method reported here provides opportunities for crop improvement of this important legume crop via genetic transformation.

Item Type:	Book Section
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Groundnut
Depositing User:	Ms Vibha Raju
Date Deposited:	13 Nov 2011 10:53
Last Modified:	13 Nov 2011 10:53
URI:	http://oar.icrisat.org/id/eprint/3448
Acknowledgement:	UNSPECIFIED
Links:	Author

View Statistics

Actions (login required)

View Item