Toomsan, B and Rupela, O P and Mittal, S and Dart, P J and Clark, K W (1984) Counting Cicer-Rhizobium using a plant infection technique. Soil Biology and Biochemistry, 16 (5). pp. 503-507. ISSN 0038-0717
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Abstract
Chickpea (Cicer arietinum L.), a large seeded pulse legume, is nodulated only by a very specific group of Rhizobium strains which do not nodulate plants other than Cicer spp. If the cotyledons are removed just after germination, subsequent seedling growth is dwarfed, and plants will reliably grow and nodulate in large test tubes (25 × 200 mm) under axenic conditions with either sand or sand + venniculite as the root medium. The dwarfed seedlings of some selected chickpea lines can be used as a “trap host” for counting Cicer-Rhizobium in pure cultures and contaminated materials by a most probable number, serial dilution-plant infection technique. The value of such a plantlet as a “trap host” for studying Cicer-Rhizobium ecology, strain authentication and inoculum quality control is demonstrated.
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Others > Food Legumes |
| Depositing User: | Mr Charan Sai Ch |
| Date Deposited: | 30 Oct 2011 13:42 |
| Last Modified: | 20 Jun 2014 04:57 |
| URI: | http://oar.icrisat.org/id/eprint/3398 |
| Official URL: | http://dx.doi.org/10.1016/0038-0717(84)90059-2 |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
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