A greenhouse screening technique to assess rust resistance in sorghum

Karunakar, R I and Pande, S and Thakur, R P (1996) A greenhouse screening technique to assess rust resistance in sorghum. International Journal of Pest Management, 42 (4). pp. 221-225. ISSN 0967-0874

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Abstract

Studies were conducted to determine the influence of plant growth stage, inoculum density, temperature, and relative humidity (RH) on development of rust (Puccinia pupurea) in sorghum (Sorghum bicolor). Rust development was maximum (>80% severity), when plants of a susceptible sorghum genotype (IS 18420) were inoculated at the four‐ to five‐leaf stage with an inoculum concentration of 4 × 106 urediniospores per ml and incubated at 20–25°C under high RH (>90%) for 24 h. Disease severity (percentage leaf area covered with rust pustules) scores were taken 2 weeks after inoculation. Using this technique, 29 sorghum genotypes were screened for rust resistance in a greenhouse. This technique proved effective In discerning resistant and susceptible genotypes, and IS 3979, ICSH 110, ICSH 86647 and ICSH 871035 were identified resistant (<20% rust severity) compared with a susceptible control IS 18420 (90% rust severity). This technique is simple and rapid, and can be used effectively and economically to screen, on a large scale, germplasm lines and breeding populations in the greenhouse.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Sorghum
Depositing User:	Mr Charan Sai Ch
Date Deposited:	29 Oct 2011 04:44
Last Modified:	29 Oct 2011 04:44
URI:	http://oar.icrisat.org/id/eprint/3314
Official URL:	http://dx.doi.org/10.1080/09670879609371999
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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