Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa

Kishore, G K and Pande, S and Podile, A R (2005) Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa. Plant Pathology, 54 (3). pp. 401-408. ISSN 0032-0862

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Abstract

Fifteen groundnut-associated bacterial isolates that inhibited by >90% the in vitro conidial germination of Phaeoisariopsis personata, causal agent of late leaf spot disease of groundnut, were applied as a prophylactic spray (108 cfu mL-1) and tested for control of the disease in the glasshouse. Two groundnut seed-associated bacterial isolates, GSE 18 and GSE 19, identified as Pseudomonas aeruginosa, reduced the lesion frequency (LF) by up to 70%. A 90-day-old peat-based formulation of P. aeruginosa GSE 18 reduced LF measured 15 days postinoculation by up to 60%. Both P. aeruginosa GSE 18 and GSE 19 were tolerant to chlorothalonil (Kavach®) up to 2000 µg mL-1 in LB broth. In glasshouse trials, GSE 18 and GSE 19 tested in combination with reduced concentrations of chlorothalonil were highly efficient in management of the disease. The disease was completely controlled by chlorothalonil (>250 µg mL-1), and in the presence of GSE 18 or GSE 19, 100 µg mL-1 chlorothalonil was equally effective. Application of rifamycin-resistant mutants of GSE 18 or GSE 19 together with chlorothalonil significantly increased the survival of these isolates in the groundnut phylloplane. In the field, a combination of GSE 18 and 500 µg mL-1 chlorothalonil reduced disease severity comparable to 2000 µg mL-1 chlorothalonil alone. Use of chlorothalonil-tolerant pseudomonads together with a quarter concentration of the recommended field dose of chlorothalonil doubled pod yield compared with the untreated unsprayed control

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Arachis hypogaea, integrated disease management, peanut, Phaeoisariopsis personata
Subjects:	Mandate crops > Groundnut
Depositing User:	Ms K Syamalamba
Date Deposited:	28 Oct 2011 04:17
Last Modified:	28 Oct 2011 04:17
URI:	http://oar.icrisat.org/id/eprint/3221
Official URL:	http://dx.doi.org/10.1111/j.1365-3059.2005.01160.x
Projects:	UNSPECIFIED
Funders:	Andhra Pradesh-Netherlands Biotechnology Program (APNLBP), Institute of Public Enterprises, Hyderabad India to ARP, and Department for International Development (DFID), UK, to SP
Acknowledgement:	This work was supported in the form of a research grant by Andhra Pradesh-Netherlands Biotechnology Program (APNLBP), Institute of Pubic Enterprises, Hyderabad, India to ARP, and Department for International Development (DFID), UK, to SP. GKK thanks the APNLBP for the award of a Senior Research Fellowship. ARP and GKK are grateful to the UGC-SAP and DST-FIST programs of the Department of Plant Sciences, University of Hyderabad, Hyderabad, for providing the research facilities
Links:	Author

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