Isolation and characterization of microsatellite markers from Musa balbisiana

Buhariwalla, H K and Jarret, R L and Jayashree, B and Crouch, J H and Ortiz, R (2005) Isolation and characterization of microsatellite markers from Musa balbisiana. Molecular Ecology Notes, 5 (2). pp. 327-330. ISSN 1471-8278

PDF - Published Version Restricted to ICRISAT users only Download (90kB) | Request a copy

Abstract

This is the first report of targeted development of B genome microsatellite markers in Musa. A total of 44 sequences with microsatellites were isolated from an enriched library of Musa balbisiana cv. 'Tani' (BB genome). Of these, 25 were polymorphic when screened on 14 diverse diploid and triploid Musa accessions. The number of alleles detected by each marker ranged between one and seven. All 25 microsatellite markers generated amplification products in all species and genome complements. These new microsatellite markers fill an important gap for diversity assessment and linkage mapping studies in plantain (AAB) and cooking banana (ABB)

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	B genome, microsatellite, Musa, polymorphism, polyploid
Subjects:	Others > Genetics and Genomics
Depositing User:	Ms K Syamalamba
Date Deposited:	28 Oct 2011 03:27
Last Modified:	28 Oct 2011 03:27
URI:	http://oar.icrisat.org/id/eprint/3220
Official URL:	http://dx.doi.org/10.1111/j.1471-8286.2005.00916.x
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors wish to dedicate this paper to the memory of Dirk R. Vuylsteke who continues to inspire us to apply our scientific endeavour for the benefit of poor farming families across the world. We gratefully acknowledge Drs V. Mahalakshmi and Isabelle Hippolyte for providing DNA samples
Links:	Author

View Statistics

Actions (login required)

View Item