AFLP Analysis of Trichoderma spp. from India Compared with Sequence and Morphological-based Diagnostics

Buhariwalla, H K and Srilakshmi, P and Kannan, S and Kanchi, R S and Chndra, S and Satyaprasad, K and Waliyar, F and Thakur, R P and Crouch, J H (2005) AFLP Analysis of Trichoderma spp. from India Compared with Sequence and Morphological-based Diagnostics. Journal Of Phytopathology, 153 (7-8). pp. 389-400. ISSN 1439-0434

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Abstract

Trichoderma species offer considerable potential for controlling aflatoxin contamination in groundnut and other crops. Initial classification of 48 Trichoderma isolates, derived from four different groundnut cultivation sites in India was based on alignment of 28S rDNA sequences to GenBank sequences of ex-type strains. This was found to be substantially more reliable than our routine morphological characterization, but did not provide a comprehensive diagnostic solution, as unique single nucleotide polymorphism (SNP) haplotypes could not be identified for all species. However, all the Trichoderma isolates could be readily distinguished by amplified fragment length polymorphism (AFLP) analysis, based on six primer pair combinations, which generated 234 polymorphic bands. In addition, individual AFLP bands were identified which differentiate closely related species. Similarly, AFLP bands were identified that correlated with different types of antagonism to Aspergillus flavus. The implications of these results for the development of simple polymerase chain reaction (PCR)-based diagnostic assays for antagonistic isolates of Trichoderma is discussed.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Trichoderma, amplified fragment length polymorphism, internal transcribed spacer, aflatoxin, groundnut
Subjects:	Mandate crops > Groundnut
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	19 Oct 2011 13:52
Last Modified:	19 Oct 2011 13:52
URI:	http://oar.icrisat.org/id/eprint/2972
Official URL:	http://dx.doi.org/10.1111/j.1439-0434.2005.00989.x
Projects:	UNSPECIFIED
Funders:	Government of UK, Government of Japan , The European Union
Acknowledgement:	This work was supported by ICRISAT core funds including ear- marked unrestricted grants from the governments of the UK, Japan and The European Union. The manuscript was greatly improved by comments from Dr John Leslie (Kansas State University). We thank B. Moss and K. Eshwar for assistance with DNA extractions, AFLP analysis and sequencing; Ramdas, Malareddy and Bukama for gen- eral laboratory assistance; P.V.N.S. Prasad and K.D.V. Prasad for assistance with figures and Dr Rolf Folkerstma (ICRISAT, Kenya) for reviewing the manuscript. The AFLP and antagonistic data used for comparative analysis in this report form part of the Doctoral thesis of Ms P. Srilakshmi.
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