Random Amplified Microsatellite Polymorphism (RAMPO) Analysis of Chickpea Accessions

    Banerjee, H and Leela, A and Seetharama, N (1998) Random Amplified Microsatellite Polymorphism (RAMPO) Analysis of Chickpea Accessions. International Chickpea Newsletter, 5. pp. 16-18. ISSN 0257-2508

    [img]
    Preview
    		 PDF - Published Version
    Download (421kB) | Preview

    Abstract

    DNA was isolated from 4 cultivated Cicer arietinum accessions and one wild C. echinospermum accession using the CTAB method. RAPD analysis with 12 primers revealed 424 amplified DNA bands. The wild species produced a maximum of 34 polymorphic bands, while all cultivars except ICCV92504 produced 2 or 3 polymorphic bands. RAMPO analysis of these accessions using microsatellite probes revealed amplified bands not visible on ethidium [homidium] bromide-stained gel. Combination of RAPD analysis and probing with microsatellite repeats (TG)10, (GATA)4 and (CAC)5 revealed polymorphic and genotype-specific unique DNA banding patterns. Thus, all 5 chickpea accessions could be distinguished using RAMPO.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Mandate crops > Chickpea
    Depositing User: Library ICRISAT
    Date Deposited: 22 Oct 2011 10:00
    Last Modified: 22 Oct 2011 10:00
    URI: http://oar.icrisat.org/id/eprint/2947
    Official URL:
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
    Links:
    View Statistics

    Actions (login required)

    View Item View Item
    OAR@ICRISAT is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.