An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea

Shivanna, K R and Saxena, N P and Seetharama, N (1997) An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea. International Chickpea Newsletter, 4. pp. 28-29. ISSN 0257-2508

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Abstract

Using pollen from a cold tolerant accession ICC8923, best results for pollen germination and pollen tube growth were obtained in medium containing 2.5% sucrose + 10% PEG + 100 mg boric acid + 300 mg calcium nitrate + 200 mg magnesium sulfate + 100 mg potassium nitrate/litre. The results of pollen germination tests using this medium (PEG medium) and Brewbaker & Kwack (BK) medium are reported. In the BK medium, pollen germination was lower and pollen tube growth was largely confined to the first 60 min with tube abnormalities such as swelling and bursting occurring in 3-h-old cultures. In PEG medium pollen germination was consistently high (<80% after 90 min) and pollen tube length was nearly five times greater than that obtained in the BK medium after 90 min.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Chickpea
Depositing User:	Library ICRISAT
Date Deposited:	26 Oct 2011 10:26
Last Modified:	26 Oct 2011 10:26
URI:	http://oar.icrisat.org/id/eprint/2855
Official URL:
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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