Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut (Arachis hypogaea L.) in India

    Satyanarayana, T and Broglio, E P and Sreenivasulu, P and Reddy, D V R and Nayudu, M V (1995) Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut (Arachis hypogaea L.) in India. Plant Pathology, 44 (6). pp. 966-970. ISSN 1365-3059

    [img] PDF - Published Version
    Restricted to ICRISAT users only
    Download (316kB) | Request a copy

    Abstract

    The double-stranded DNA of the chlorotic vein banding isolate of peanut chlorotic streak caulimovirus (PC1SV-CVB), isolated from purified virus, resolved into circular and linear molecules similar to those of other caulimoviruses. A physical map of viral DNA was constructed, which showed the PCLSV-CVB DNA to be circular and composed of approximately 8.2 kbp. A number of restriction sites were found to be shared with a similar caulimovirus, PCLSV. Nevertheless, several differences between physical maps of the 2 viruses suggested that PCLSV-CVB should be considered as a distinct strain of PCLSV. Bam HI-cleaved PCLSV-CVB DNA was cloned into pUC 118 and was infectious when cleaved from the cloning vector and inoculated onto Vigna unguiculata [cowpeas].

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Mandate crops > Groundnut
    Depositing User: Library ICRISAT
    Date Deposited: 27 Oct 2011 05:10
    Last Modified: 27 Oct 2011 05:11
    URI: http://oar.icrisat.org/id/eprint/2820
    Official URL: http://dx.doi.org/10.1111/j.1365-3059.1995.tb02654...
    Projects: UNSPECIFIED
    Funders: Council of Scientific and Industrial Research(India)
    Acknowledgement: The results presented in this paper were submitted as ICRISAT Journal Article No. 1601. T.S. thanks the Council of Scientific and Industrial Research, New Delhi, India, for providing a fellowship (in the form of CSIRSRF) during the period of research. The authors thank Dr R,J. Shepherd. University of Kentucky, Lexington, USA and Dr M.A. Mayo. Scottish Crop Research Institute, Invergowrie, Dundee, Scotland, UK, for critically reading the manuscript, and Dr R.D. Richins, University of California, Riverside, USA for generously providing the CaMV DNA
    Links:
    View Statistics

    Actions (login required)

    View Item View Item
    OAR@ICRISAT is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.