A Technique for Phytophthora drechsleri f. sp. cajani Sporangia and Zoospores Production

Kannaiyan, J and Sheila, V K and Nene, Y L (1994) A Technique for Phytophthora drechsleri f. sp. cajani Sporangia and Zoospores Production. International Chickpea Newsletter, 1. pp. 36-37. ISSN 0257-2508

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Abstract

Cultures on V-8 juice agar medium were incubated for 5 d at 30°C, then 5 mm diam. discs were removed from the periphery of the colonies and floated in clear V-8 juice and sterile distilled water (1:5) for a further 24 h at 30° under fluorescent light. The mycelial discs were rinsed twice in sterile distilled water and incubated again under the same conditions in sterile distilled water. Abundant sporangia were produced in 16 h and suspensions of c. 50 000 zoospores/ml were obtained.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Subjects: Mandate crops > Pigeonpea
Others > Fertilizer Applications
Depositing User: Library ICRISAT
Date Deposited: 27 Oct 2011 06:03
Last Modified: 27 Oct 2011 06:03
URI: http://oar.icrisat.org/id/eprint/2811
Official URL:
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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