A new source of cytoplasmic male sterility in pearl millet: RFLP analysis of mitochondrial DNA.

Sujata, V and Sivaramakrishnan, S and Rai, K N and Seetha, K (1994) A new source of cytoplasmic male sterility in pearl millet: RFLP analysis of mitochondrial DNA. Genome, 37 (3). pp. 482-486. ISSN 0831-2796

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Abstract

A new source of cytoplasmic male sterility (cms) in pearl millet (Pennisetum glaucum (L.) R.Br.) derived from a half-sib progeny of the Early Gene Pool (EGP 261) and used in a male-sterile line, ICMA 90111, was compared with other known cms sources for RFLP of mitochondrial (mt) DNA. Southern blot hybridization of mtDNA from ICMA 90111 digested with several restriction enzymes and probed with homologous mtDNA clones from pearl millet and heterologous gene clones from maize and wheat revealed the RFLP patterns of ICMA 90111 distinct from others studied so far. The dendrogram of male-sterile lines constructed from the Southern blot hybridization patterns indicated that ICMA 90111 represents a separate group. Our results suggest that this source of cms is unique in several respects.Key words: Pennisetum glaucum, cytoplasmic male sterility, mitochondrial DNA, RFLP.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Millets
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	15 Oct 2011 08:01
Last Modified:	15 Oct 2011 08:01
URI:	http://oar.icrisat.org/id/eprint/2602
Official URL:	http://dx.doi.org/10.1139/g94-067
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	We thank Mr.R.Luke for technical assistance.
Links:	Author

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