Evaluation of the shoot regeneration response in tissue culture of pigeonpea (Cajanus cajan [L.] Millsp.) varieties adapted to eastern and southern Africa

de Villiers, S and Emongor, Q and Njeri, R and Gwata, E and Hoisington, D A and Njagi, I and Silim, S and Sharma, K K (2008) Evaluation of the shoot regeneration response in tissue culture of pigeonpea (Cajanus cajan [L.] Millsp.) varieties adapted to eastern and southern Africa. African Journal of Biotechnology, 7 (5). pp. 587-590. ISSN 1684-5315

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Abstract

Seven varieties of pigeonpea (Cajanus cajan [L.] Millsp.) of varying growth durations and adapted to a wide range of environments across eastern and southern Africa were evaluated for their shoot regeneration response in tissue culture. On a standardized shoot regeneration medium, the short duration varieties (ICPV 88091 and ICPV 86012) generally responded faster and better than the medium duration (ICEAP 00554 and ICEAP 00557) and long duration (ICEAP 00020, ICEAP 00040 and ICEAP 00053) varieties. However, all the tested varieties produced healthy rooted plants in vitro that could be transferred to the greenhouse where they exhibited normal growth, flowering and viable seed set. This study established the basis for genetic engineering of African pigeonpea varieties.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Shoot regeneration, pigeonpea, Cajanus cajan, African varieties.
Subjects: Mandate crops > Pigeonpea
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 14 Oct 2011 08:24
Last Modified: 07 Dec 2011 06:08
URI: http://oar.icrisat.org/id/eprint/2504
Official URL:
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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