In vivo inhibition of Helicoverpa armigera gut pro-proteinase activation by non-host plant protease inhibitors

Parde, Vinod Dadarao and Sharma, H C and Kachole, M S (2010) In vivo inhibition of Helicoverpa armigera gut pro-proteinase activation by non-host plant protease inhibitors. Journal of Insect Physiology, 56 (9). pp. 1315-1324. ISSN 0022-1910

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Abstract

We evaluated 22 different host and non-host plant protease inhibitors (PIs) for in vivo inhibition of Helicoverpa armigera gut pro- and proteinases, and their biological activity against the pod borer, H. armigera, the most important pest of agriculture and horticultural crops worldwide. In vitro activation of H. armigera gut pro-proteinases (HaGPPs) in larvae fed on non-host plant PIs showed significant in vivo inhibition of HaGPPs activation in solution as well as in gel assays. The larvae fed on diet incorporated with Datura alba ness PIs showed highest inhibition of HaGPPs, followed by Psophocarpus tetragonolobus. Non-host plant PIs from Pongamia pinnata, Mucuna pruriens, Capsicum annuum, and Nigela sativa showed maximum inhibitory potential towards HaGPs in vivo, and also exhibited moderate level of inhibition of pro-proteinases. However, some of non-host plant PIs, such as those from Penganum harmala and Solanum nigrum, and the principal host plant PIs, viz., Cicer arietinum and Cajanus cajan did not inhibit HaGPP activity. Pro-proteinase level increased with the growth of the larvae, and maximum HaGPP activity was observed in the fifth-instars. Larvae fed on diets with D. alba ness PIs showed greater inhibition of HaGPPs as compared to the larvae fed on diets with P. tetragonolobus. Low concentrations of partially purified HaGPs treated with gut extract of larvae fed on D. alba ness showed that out of 10 proteinase isoforms, HaGPs 5 and 9 were activators of pro-proteinases. Larval growth and development were significantly reduced in the larvae fed on the non-host plant PIs, of which D. alba ness resulted in highest stunted growth of H. armigera larvae. The in vivo studies indicated that non-host plant PIs were good candidates as inhibitors of the HaGPs as well as HaGPPs. The PIs from the non-host plants can be expressed in genetically engineered plants to confer resistance to H. armigera.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Others > Entomology
Depositing User:	Library ICRISAT
Date Deposited:	17 Aug 2011 08:28
Last Modified:	08 Jun 2012 08:01
URI:	http://oar.icrisat.org/id/eprint/250
Official URL:	http://dx.doi.org/10.1016/j.jinsphys.2010.04.003
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors would like to thank the staff of entomology for supplying Helicoverpa armigera culture for bioassays. We also thank Dr. M.K. Dhillon, Senior Scientist, IARI, New Delhi, for his valuable comments on the manuscript.
Links:	Author

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