Biotechnology and chickpea breeding

    Rheenen, H A van (1992) Biotechnology and chickpea breeding. International Chickpea Newsletter, 26. pp. 14-17.

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    Abstract

    Isoenzyme, RFLP and randomly amplified polymorphic DNA (RAPD) analyses were carried out on 2 sets of chickpea (Cicer arietinum) varieties. Isoenzyme analysis of a number of enzymes in both sets detected only one polymorphism, for alanyl naphthlyamide aminopeptidase, RFLP analysis failed to show polymorphism, while RAPD analysis successfully detected varietal differences and gave sharp banding patterns; DNA of 3 of the 8 varieties in one set showed polymorphism when hybridized with 14 different primers containing 10 bases each, while all 4 primers used for the second set of varieties showed polymorphism. Thus, these primers with short sequences were able to detect genomic variations at relatively high frequency.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Mandate crops > Chickpea
    Depositing User: Library ICRISAT
    Date Deposited: 13 Oct 2011 11:04
    Last Modified: 13 Oct 2011 11:04
    URI: http://oar.icrisat.org/id/eprint/2451
    Official URL:
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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