Introgression of Cajanus platycarpus genome into cultivated pigeonpea, C. cajan

    Mallikarjuna, N and Jadhav, D R and Reddy, P (2006) Introgression of Cajanus platycarpus genome into cultivated pigeonpea, C. cajan. Euphytica, 149 (1-2). pp. 161-167. ISSN 0014-2336

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    Abstract

    Cajanus platycarpus, an incompatible wild species from the tertiary gene pool of pigeonpea (C. cajan (L.) Millspaugh), has many desirable characteristics for the improvement of cultivated varieties. To necessitate such transfers, embryo rescue techniques were used to obtain F1 hybrids. The F1 hybrids were treated with colchicine to obtain tetraploid hybrids, that were selfed to obtain F2, F3 and F4 progenies. All of the hybrids and subsequent progenies had an intermediate morphology between the two parents. Backcrossing of the tetraploid hybrids with cultivated pigeonpea was not possible given embryo abortion, with smaller aborted embryos than those obtained in the F0 parental cross. As a route of introgression, diploid F1 hybrids were backcrossed with cultivated pigeonpea and BC1 progeny obtained by in vitro culture of aborting embryos. BC2 plants were obtained by normal, mature seed germination. Although embryo rescue techniques had to be used to obtain F1 and BC1 plants, it was possible to produce BC2 and subsequent generations through direct mature seed. Every backcross to cultivated pigeonpea increased pollen fertility and the formation of mature seeds.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Uncontrolled Keywords: Cajanus platycarpus, Cajanus cajan, pigeonpea, interspecific hybridization, embryo rescue, diploid, tetraploid
    Subjects: Mandate crops > Pigeonpea
    Depositing User: Mr Sanat Kumar Behera
    Date Deposited: 10 Oct 2011 10:36
    Last Modified: 22 Nov 2011 04:42
    URI: http://oar.icrisat.org/id/eprint/2197
    Official URL: http://dx.doi.org/10.1007/s10681-005-9063-6
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: The authors thank the AP-Netherlands Biotechnology project for funds to carry out research
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