Interspecific hybridization between Cicer arietinum and C. pinnatifidum

Badami, P S and Mallikarjuna, N and Moss, J P (1997) Interspecific hybridization between Cicer arietinum and C. pinnatifidum. Plant Breeding, 116 (4). pp. 393-395. ISSN 1439-0523

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Abstract

An interspecific hybrid between Cicer arietinum cv. GL 769 and a wild species C. pinnatifidum was obtained after emasculation, pollination and application of growth regulators. Ovules were cultured and embryos were later dissected to obtain hybrid plants. These plants were albinos and morphologically resembled C. pinnatifidum. Shrivelled seeds were also obtained in 2% of the crosses, which on germination gave rise to albino plants. These plants did not survive beyond 20 days. The hybrid nature of these plants was confirmed by esterase isozyme studies. Hybrid shoots obtained from germinating embryos were cultured on modified ML-6 medium with BAP 2 mg/1, IAA 0.5 mg/1, where they turned green after 3–4 weeks. Transmission electron microscopy (TEM) studies on leaf sections from green hybrid shoots showed an improvement in the chloroplast structure, with better organized grana.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Cicer arietinum, C. pinnatifidum, chloroplast, embryo rescue, grafting, interspecific hybridization, ovule culture
Subjects:	Mandate crops > Chickpea
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	10 Oct 2011 10:25
Last Modified:	20 Jan 2012 02:59
URI:	http://oar.icrisat.org/id/eprint/2194
Official URL:	http://dx.doi.org/10.1111/j.1439-0523.1997.tb01019...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors are grateful to the ICRISAT Asia Centre GeneticResources Division for supply of wild Cicer germplasm. Genetic Enhancement Division forfieldfacilities and to A. K. Murthy, ElectronMicroscope Unit for TEM studies.
Links:	Author

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