Detached leaf assay to evaluate transgenic pigeonpea plants for resistance to Helicoverpa armigera

Gopal Swamy, S V S and Sharma, H C and Sharma, K K and Subbaratnam, G V and Kumar, C S (2007) Detached leaf assay to evaluate transgenic pigeonpea plants for resistance to Helicoverpa armigera. Resistant Pest Management Newsletter, 16 (2). pp. 24-27. ISSN 1061-7795

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Abstract

Introduction of transgenic insect-resistant pigeonpea (Cajanus cajan (L.) Millsp.), is expected to be useful in minimizing Helicoverpa armigera (Hubner) damage, which is the major constraint for its production. In this context, it is important to develop techniques to evaluate effectiveness of transgenic plants for resistance to H. armigera. Therefore, we evaluated the usefulness of detached leaf assay to assess the efficacy of transgenic pigeonpea (var; ICPL 88039 and ICPL 87) plants carrying Btcry1Ab and SBTI genes for resistance to H. armigera. The levels of Cry1Ab or SBTI proteins in the transgenic pigeonpea plants were not sufficient to cause significant deterrent effects on leaf feeding, larval survival, and larval weight of H. armigera. However, detached leaf assay was found to be quite useful for evaluation of transgenic pigeonpea plants for resistance to H. armigera.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Pigeonpea
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	08 Oct 2011 12:22
Last Modified:	08 Oct 2011 12:22
URI:	http://oar.icrisat.org/id/eprint/2083
Official URL:	http://whalonlab.msu.edu/Newsletter/index.html
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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